Shimoyama Yuka, Fujii Makiko, Kanda Yoshimi, Mizoguchi Asako, Oda Hiroshi, Koizumi Naoya, Watanabe Yoshiteru
Showa Pharmaceutical University, Tokyo 194-8543, Japan.
Chem Pharm Bull (Tokyo). 2010 Mar;58(3):429-31. doi: 10.1248/cpb.58.429.
We investigated the skin penetration of liposomes under two different application conditions; occluded and large application amount (1 ml/cm(2)), and open and small application amount (10 mul/cm(2)). Liposomes containing fluorescence-labeled phospholipids or carboxyfluorescein (CF) were used. In application under occluded conditions, phospholipids showed no penetration, even in the stratum corneum (SC). CF penetration in the skin after application of liposome was no different that after application of CF solution. In contrast, phospholipids penetrated the skin, particularly the SC and hair follicles, under open conditions. CF in liposome showed enhanced penetration in the SC and epidermis, but not in the dermis. On observation of the drying process, CF recrystallized from solution, but this did not occur with CF incorporated into liposome. It is possible that crystallization of CF is prevented by encapsulation in liposome, or that penetration occurs more readily with liposome.
封闭且大量涂抹（1毫升/平方厘米），以及开放且少量涂抹（10微升/平方厘米）。使用了含有荧光标记磷脂或羧基荧光素（CF）的脂质体。在封闭条件下涂抹时，即使在角质层（SC）中，磷脂也没有渗透。涂抹脂质体后皮肤中CF的渗透与涂抹CF溶液后没有差异。相比之下，在开放条件下磷脂会渗透到皮肤中，尤其是角质层和毛囊。脂质体中的CF在角质层和表皮中的渗透增强，但在真皮中没有。在观察干燥过程时，CF从溶液中重结晶，但包裹在脂质体中的CF不会发生这种情况。有可能是脂质体的包裹防止了CF的结晶，或者是脂质体更容易发生渗透。
