Omelianenko N P
Central Institute of Traumatology and Orthopaedics, Moscow, USSR.
Clin Orthop Relat Res. 1991 May(266):34-41.
Extracellular components of human articular cartilage have been studied using quantitative and ultrastructural methods. A new combination of electron microscopic, physicochemical, and mathematical methods has been proposed to study the structural organization of the fibrillar stroma, interfibrillar spaces, and structural proteoglycans. The new methodology has made it possible to isolate in the cartilage three layers with various types of spatial orientation of the fibrillar stroma. The summary volume and differential distribution of the interstitial microcirculatory channel have been derived. Proteoglycans of the integrative buffer metabolic medium have been identified using transmission and scanning electron microscopy. The data suggest a more accurate concept of the structural organization of human articular cartilage.
已使用定量和超微结构方法对人关节软骨的细胞外成分进行了研究。有人提出了一种将电子显微镜、物理化学和数学方法相结合的新方法，用于研究纤维基质、纤维间空间和结构蛋白聚糖的结构组织。这种新方法使得在软骨中分离出具有不同纤维基质空间取向类型的三层结构成为可能。已得出间质微循环通道的总体积和差异分布。已使用透射电子显微镜和扫描电子显微镜鉴定了整合缓冲代谢介质中的蛋白聚糖。这些数据提出了关于人关节软骨结构组织的更准确概念。
