Hu Sen, Che Jin-wei, DU Ying, Bao Cheng-mei
First Hospital affiliated to the PLA General Hospital, Beijing 100048, China.
Zhonghua Shao Shang Za Zhi. 2009 Dec;25(6):451-3.
To investigate the effect of vitamin C (VC) on alleviating peroxidative damage in gut of dogs during enteral fluid resuscitation of severe burn shock.
Eighteen male Beagle dogs were subjected to 50% total body surface area (TBSA) full-thickness burn 24 hours after duodenostomy and cannulation of cervical artery and vein. The dogs were divided into no resuscitation (NR) group (no treatment after burn), enteral resuscitation (ER) group, and ER+VC group according to the random number table, with 6 dogs in each group. Dogs in ER and ER+VC groups were respectively infused with glucose-electrolyte solution (GES) and GES containing 250 mg/kg VC through duodenostomy tube 30 minutes after burn. The infusion rate and volume of GES were in accordance with Parkland formula. Venous blood of dogs was drawn before (0) and at 2, 4, 6, and 8 post burn hours (PBH) to determine the activity of diamine oxidase (DAO) in plasma. Dogs were sacrificed at PBH 8 to collect specimens of jejunum tissue for determining the content of malondialdehyde (MDA), and activity of myeloperoxidase (MPO), xanthine oxidase (XOD) and superoxide dismutase (SOD), and assessment of the water ratio of intestinal tissue by dry-wet weight method.
50% TBSA burn injury resulted in significant elevation of DAO in every group. The activity of DAO in ER group was obviously higher than that in NR group at PBH 6 and 8 (P < 0.05), but DAO activity in ER+VC group was significantly lower than those in the other two groups after PBH 2 (P < 0.05 or P < 0.01). MDA content, MPO and XOD activity and the water ratio of intestinal tissue [(5.74 +/- 0.51) nmol/mg, (2.08 +/- 0.46) U/g, (58.4 +/- 3.8) U/mg, (81.5 +/- 1.8)%] in ER group at PBH 8 was respectively significantly higher than that in NR group [(5.43 +/- 0.25) nmol/mg, (1.55 +/- 0.21) U/g, (50.1 +/- 2.8) U/mg, (78.3 +/- 1.5)%, P < 0.05 or P < 0.01]. While the activity of SOD in ER group (72 +/- 12) U/mg was lower than that in NR group (97 +/- 20) U/mg. MDA content, MPO and XOD activity and water ratio of intestinal tissue in ER+VC group was respectively lower than that in ER group, with activity of SOD in the former group higher than that in the latter group (P < 0.01).
Vitamin C can alleviate peroxidative damage and tissue edema in gut induced by ischemia and reperfusion, and intestinal complications during oral rehydration during burn shock can be reduced.
探讨维生素C（VC）对严重烧伤休克肠内液体复苏期间减轻犬肠道过氧化损伤的作用。
18只雄性比格犬在十二指肠造口术及颈动静脉插管24小时后，给予50%总体表面积（TBSA）全层烧伤。根据随机数字表将犬分为未复苏（NR）组（烧伤后未治疗）、肠内复苏（ER）组和ER + VC组，每组6只犬。ER组和ER + VC组犬在烧伤后30分钟分别通过十二指肠造口管输注葡萄糖电解质溶液（GES）和含250 mg/kg VC的GES。GES的输注速率和量按照Parkland公式。在烧伤前（0）及烧伤后2、4、6和8小时（PBH）采集犬静脉血，测定血浆中二胺氧化酶（DAO）活性。在PBH 8处死犬，收集空肠组织标本，测定丙二醛（MDA）含量、髓过氧化物酶（MPO）、黄嘌呤氧化酶（XOD）和超氧化物歧化酶（SOD）活性，并采用干湿重法评估肠组织的含水量。
50% TBSA烧伤导致每组犬DAO显著升高。PBH 6和8时，ER组DAO活性明显高于NR组（P < 0.05），但PBH 2后ER + VC组DAO活性显著低于其他两组（P < 0.05或P < 0.01）。PBH 8时，ER组肠组织MDA含量、MPO和XOD活性及含水量[（5.74 ± 0.51）nmol/mg，（2.08 ± 0.46）U/g，（58.4 ± 3.8）U/mg，（81.5 ± 1.8）%]分别显著高于NR组[（5.43 ± 0.25）nmol/mg，（1.55 ± 0.21）U/g，（50.1 ± 2.8）U/mg，（78.3 ± 1.5）%，P < 0.05或P < 0.01]。而ER组SOD活性（72 ± 12）U/mg低于NR组（97 ± 20）U/mg。ER + VC组肠组织MDA含量、MPO和XOD活性及含水量分别低于ER组，前者SOD活性高于后者（P < 0.01）。
维生素C可减轻缺血再灌注诱导的肠道过氧化损伤和组织水肿，并可减少烧伤休克口服补液期间的肠道并发症。
