Cao Jing-Rong, Wei Xing, Yan Zhong-Qiang, Shen Ding-Xia, Luo Yan-Ping
Department of Microbiology, PLA General Hospital, Beijing 100853, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2009 Aug;30(8):832-5.
To investigate antibiotic resistance, carbapenemase genotype and the molecular epidemiology of multidrug-resistant Acinetobacter baumannii (Aba) collected from 3 military hospitals in China.
The minimum inhibitory concentrations (MIC) were examined by ager dilution method. Genotypes of carbapenemases were amplified by multiplex PCR and its products were sequenced. PCR was used to detect per gene. Homology of the resistant isolates was analyzed by pulse-field gel electrophoresis (PFGE).
Among the 64 MDRA strains, 78.1% (50) strains possessed bla(OXA-23) gene, 89.1% (57) carried Class 1 integrase gene, 39.1% (25) with bla(PER-1) gene, and 1 strain with bla(OXA-58-like) gene. PFGE showed that 13 (A, B, C, D, E genotype) different clones were identified in these strains. A, B, and U clones were the predominant clones in three hospitals, respectitively.
Outbreaks of multidrug-resistant Aba occurred at 3 military hospitals with the most prevalent carbapenemase as OXA-23 enzyme. OXA-58 type of carbapenemase and per-1 in Aba were also isolated.
调查从中国3家军队医院收集的多重耐药鲍曼不动杆菌（Aba）的抗生素耐药性、碳青霉烯酶基因型及分子流行病学特征。
采用琼脂稀释法检测最低抑菌浓度（MIC）。通过多重PCR扩增碳青霉烯酶基因型并对其产物进行测序。采用PCR检测每个基因。通过脉冲场凝胶电泳（PFGE）分析耐药菌株的同源性。
在64株多重耐药Aba菌株中，78.1%（50株）菌株携带bla(OXA-23)基因，89.1%（57株）携带1类整合酶基因，39.1%（25株）携带bla(PER-1)基因，1株携带bla(OXA-58-like)基因。PFGE显示这些菌株中鉴定出13种（A、B、C、D、E基因型）不同克隆。A、B和U克隆分别是3家医院中的主要克隆。
3家军队医院发生了多重耐药Aba的暴发，最常见的碳青霉烯酶为OXA-23酶。还分离出了Aba中的OXA-58型碳青霉烯酶和per-1。
