Liu Jian, Yang Xiao-ming, Liu Gang, Chang Lian-sheng, Zhang Li-rong, Song Dong-kui
Department of Urinary Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Zhonghua Yi Xue Za Zhi. 2009 Dec 1;89(44):3122-5.
To investigate the association between genetic polymorphism of UGT1A7 and susceptibility of bladder cancer.
Based upon a case-control study, UGT1A7 polymorphisms were determined by the semi-nested polymerase chain reaction (SN-PCR) and allele-specific polymerase chain reaction (AS-PCR) in 208 cases with bladder cancer and 205 non-tumor controls. Risks were evaluated by unconditional logistic regression analysis.
The frequency of variant homozygous genotype in cases (20.7%) was higher than that in controls (12.2%) and the difference was statistically significant [P < 0.05, OR = 2.16 (1.18 - 3.96)]. The frequency of variant allele (*)3 in cases was higher than that in controls (27.9%, 20.5% respectively) and the difference was statistically significant [P = 0.009, OR = 1.56 (95%CI: 1.12 - 2.18)]. The smokers with variant homozygous and heterozygous genotypes showed an increased risk of bladder cancer compared with those with wild genotype [2.16 (95%CI: 1.07 - 4.36), 2.64 (95%CI: 1.02 - 6.80) respectively]. There was no association between the UGT1A7 polymorphisms and the pathological grade and clinical stage of bladder cancer (both P > 0.05).
The genetic polymorphisms of UGT1A7 are associated with the susceptibility of bladder cancer and have interactions with smoking in bladder carcinogenesis.
探讨尿苷二磷酸葡萄糖醛酸基转移酶1A7（UGT1A7）基因多态性与膀胱癌易感性之间的关系。
基于病例对照研究，采用半巢式聚合酶链反应（SN-PCR）和等位基因特异性聚合酶链反应（AS-PCR）对208例膀胱癌患者和205例非肿瘤对照者的UGT1A7基因多态性进行检测。通过非条件逻辑回归分析评估风险。
病例组变异纯合基因型频率（20.7%）高于对照组（12.2%），差异有统计学意义[P<0.05，比值比（OR）=2.16（1.18 - 3.96）]。病例组变异等位基因（*）3频率高于对照组（分别为27.9%、20.5%），差异有统计学意义[P = 0.009，OR = 1.56（95%可信区间：1.12 - 2.18）]。与野生基因型吸烟者相比，变异纯合和杂合基因型吸烟者患膀胱癌的风险增加[分别为2.16（95%可信区间：1.07 - 4.36），2.64（95%可信区间：1.02 - 6.80）]。UGT1A7基因多态性与膀胱癌的病理分级和临床分期均无相关性（均P>0.05）。
UGT1A7基因多态性与膀胱癌易感性相关，且在膀胱癌发生过程中与吸烟存在交互作用。
