Duan Zheng-xiu, Li Ming-hui, Yu Juan, Wang Wen, Guo Wen-ping, Zhang Yong-zhen
College of Life Science, Shihezi University, Shihezi 832003, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2009 Oct;30(10):1047-50.
Comparing the difference of virulence between the strain CGRn5310 (HTNV) and the strain HR54 (SEOV) isolated both from Rattus norvegicus.
Suckling mice were used to compare the difference of virulence between the two strains. Hantavirus antigens were detected in brain and lung tissues collected from the infected mice.
Compared with the control group, all infected mice grew slowly. Furthermore, the mice inoculated intracerebrally with either CGRn5310 or HR45 appeared ruffled fur, and reduced activity, followed by neurological symptoms, such as paralyses and convulsions. The half lethal dose (LD(50)) of CGRn5310 strain was 10(-6.42), whereas the LD(50) of HR54 strain was 10(-4.51). Hantavirus antigens were identified in brain and lung tissues from the mice infected with the strain CGRn5310 and the strain HR54.
LD(50) of the strain CGRn5310 was significantly higher than that of the strain HR54. Our results suggested that the virulence of the spillover hantavirus might only slightly be influenced by the non-reservoir rodents.
比较从褐家鼠分离出的CGRn5310株（汉坦病毒）和HR54株（汉城病毒）之间的毒力差异。
使用乳鼠比较这两种毒株之间的毒力差异。从感染小鼠收集的脑和肺组织中检测汉坦病毒抗原。
与对照组相比，所有感染小鼠生长缓慢。此外，脑内接种CGRn5310或HR45的小鼠出现被毛蓬乱、活动减少，随后出现神经症状，如麻痹和惊厥。CGRn5310株的半数致死剂量（LD(50)）为10(-6.42)，而HR54株的LD(50)为10(-4.51)。在感染CGRn5310株和HR54株的小鼠的脑和肺组织中鉴定出汉坦病毒抗原。
CGRn5310株的LD(50)显著高于HR54株。我们的结果表明，溢出性汉坦病毒的毒力可能仅受到非储存宿主啮齿动物的轻微影响。
