Hua Ben-lin, Fu Xin-ge, Hu Wen-hao, Yin Liang, Kang Xue-ling, Li Hong-an, Jiang Jin-fang, Li Feng
Department of Pathology, Shihezi University School of Medicine, Shihezi 832002, China.
Zhonghua Bing Li Xue Za Zhi. 2009 Dec;38(12):806-9.
To explore the Notch1 mRNA and protein expression in human breast cancers and normal mammary tissues, and their relationship with the clinical indicators of breast cancers were analyzed.
Notch1 gene of human breast invasive ductal carcinoma (IDC) and normal mammary gland tissues were amplified by RT-PCR, and the expression of Notch1 protein was detected by immunohistochemical Streptavidin-Biotin Complex (SP) stain in 60 IDC, 30 ductal carcinoma in situ (DCIS) and 60 normal mammary tissues.
Notch1 gene of human IDC and normal mammary tissues both could express in a transcription level; the positive rates of Notch1 protein expression in normal mammary tissues and DCIS were 55% and 70%. Respectively, which did not differ statistically (P > 0.05), while the positive rate in IDC was 90%, significantly higher than that of the normal mammary tissues and DCIS (P < 0.05). The high expression of Notch1 protein in IDC correlate significantly with lymph node metastasis, pathological grades and TNM stages.
Notch1 protein was over expressed in breast IDC. A high Notch1 protein expression is considered associating with the evolution and malignant transformation of the breast tumor. The expression of Notch1 gene maybe impact the effect of on the progression of breast cancers.
探讨Notch1 mRNA及蛋白在人乳腺癌组织和正常乳腺组织中的表达情况，并分析其与乳腺癌临床指标的关系。
采用逆转录聚合酶链反应（RT-PCR）扩增人乳腺浸润性导管癌（IDC）组织和正常乳腺组织中的Notch1基因，采用免疫组织化学链霉亲和素-生物素复合物（SP）法检测60例IDC、30例导管原位癌（DCIS）及60例正常乳腺组织中Notch1蛋白的表达。
人IDC组织和正常乳腺组织中的Notch1基因均能在转录水平表达；正常乳腺组织和DCIS中Notch1蛋白表达阳性率分别为55%和70%，差异无统计学意义（P>0.05），而IDC中Notch1蛋白表达阳性率为90%，显著高于正常乳腺组织和DCIS（P<0.05）。IDC中Notch1蛋白高表达与淋巴结转移、病理分级及TNM分期显著相关。
Notch1蛋白在乳腺IDC中呈过表达。Notch1蛋白高表达被认为与乳腺肿瘤的演进和恶性转化有关。Notch1基因的表达可能影响乳腺癌的进展。
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