Yang Ming, Fan Dong-Mei, Gao Ying-Dai, Zhou Yuan, Ji Qing, Shao Xiao-Feng, Wang Jin-Hong, Xu Yuan-Fu, Xiong Dong-Sheng, Yang Chun-Zheng
State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, CAMS & PUMC, Tianjin 300020, China.
Zhonghua Xue Ye Xue Za Zhi. 2009 Dec;30(12):812-5.
To investigate the role of Ara-C in regulating anti-CD3/anti-Pgp mediating T-lymphocytes activities against multi-drug resistant leukemia cells.
The diabody of anti-CD3/anti-Pgp was purified by E-tag affinity chromatography. K562 and K562/A02 cells were treated with Ara-C. The expressions of B7-1 and B7-2 on K562 and K562/AO2 cells were detected by FACS. The cytotoxicity of T-lymphocytes combined with anti-CD3/anU-Pgp plus Ara-C was analyzed by CytoTox 96 nonradioactive method.
The expressions of B7-1 and B7-2 on K562 and K562/A02 cells treated by Ara-C was significantly higher than those untreated. The effect/target ratio was from 0.39:1 to 25:1, and the killing rate of activated T cells to anti-drug-resistant leukemia cells was from (16.44 +/- 1.20)% to (60.49 +/- 2.90)%. The killing rates were increased gradually, with both the effect/target ratio and the antibody concentration increasing (P < 0.05).
Ara-C may be an important adjuvant for improving anti-CD3/anti-Pgp mediating T-lymphocytes activities against multi-drug resistant leukemia cells.
探讨阿糖胞苷（Ara-C）在调节抗CD3/抗P糖蛋白介导的T淋巴细胞对多药耐药白血病细胞活性中的作用。
采用E标签亲和层析法纯化抗CD3/抗P糖蛋白双抗体。用阿糖胞苷处理K562和K562/A02细胞。通过流式细胞术检测K562和K562/AO2细胞上B7-1和B7-2的表达。采用CytoTox 96非放射性方法分析T淋巴细胞联合抗CD3/抗P糖蛋白加阿糖胞苷的细胞毒性。
阿糖胞苷处理的K562和K562/A02细胞上B7-1和B7-2的表达明显高于未处理的细胞。效靶比为0.39:1至25:1，活化T细胞对耐药白血病细胞的杀伤率为(16.44±1.20)%至(60.49±2.90)%。随着效靶比和抗体浓度的增加，杀伤率逐渐升高（P<0.05）。
阿糖胞苷可能是提高抗CD3/抗P糖蛋白介导的T淋巴细胞对多药耐药白血病细胞活性的重要佐剂。
