Said Ali K, Ferencz Agnes, Nemcsók J, Hermesz Edit
Department of Biochemistry and Molecular Biology, Faculty of Sciences, University of Szeged, Szeged, Hungary.
Acta Biol Hung. 2010 Mar;61(1):10-23. doi: 10.1556/ABiol.61.2010.1.2.
Heat shock proteins (HSPs) and metallothioneins (MTs) play important roles in protection against environmental stressors. The present study analyzes and compares the regulation of heat shock ( hsp70, hsc70-1 and hsp90alpha ) and metallothionein (MT-1 and MT-2) genes in the heart of common carp, in response to elevated temperature, cold shock and exposure to several heavy metal ions (As 3+ , Cd 2+ and Cu 2+ ), in whole-animal experiments. Among these metals, arsenate proved to be the most potent inducer of the examined stress genes; the hsp90alpha and MT-1 mRNA levels were elevated 11- and 10-fold, respectively, after a 24-h exposure. In contrast, Cd 2+ at 10 mg/L had no impact on the expression of hsp90alpha , and the MT genes also proved to be rather insensitive to Cd 2+ treatment in the heart: only a 2-2.5-fold induction was observed in response to 10 mg/L Cd 2+ . Heat shock resulted in a transient induction of hsp70 (19-fold) and hsp90alpha (15-fold), while elevated temperature had no effect on the expression of the MTs. Direct cold shock induced hsp70 expression (14-fold), while the hsp90alpha (26-fold) and MT-2 (2-fold) expressions peaked after the recovery period following a direct cold shock. The five stress genes examined in this study exhibited a unique, tissue-specific basal expression pattern and a characteristic sensitivity to metal treatments and temperature shocks.
热休克蛋白（HSPs）和金属硫蛋白（MTs）在抵御环境应激源方面发挥着重要作用。本研究在整体动物实验中，分析并比较了鲤鱼心脏中热休克（hsp70、hsc70 - 1和hsp90α）和金属硫蛋白（MT - 1和MT - 2）基因对温度升高、冷休克以及暴露于几种重金属离子（As³⁺、Cd²⁺和Cu²⁺）的调控情况。在这些金属中，砷酸盐被证明是所检测应激基因的最有效诱导剂；暴露24小时后，hsp90α和MT - 1的mRNA水平分别升高了11倍和10倍。相比之下，10 mg/L的Cd²⁺对hsp90α的表达没有影响，MT基因在心脏中对Cd²⁺处理也相当不敏感：在10 mg/L Cd²⁺处理下仅观察到2 - 2.5倍的诱导。热休克导致hsp70（19倍）和hsp90α（15倍）的瞬时诱导，而温度升高对MTs的表达没有影响。直接冷休克诱导hsp70表达（14倍），而hsp90α（26倍）和MT - 2（2倍）的表达在直接冷休克后的恢复期达到峰值。本研究中检测的五个应激基因表现出独特的、组织特异性的基础表达模式以及对金属处理和温度休克的特征性敏感性。
