Oxidative Stress and Cell Signaling Research Group, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
Departamento de Medicina-Núcleo de Saúde, Universidade Federal de Rondônia, 76801-059 Porto Velho, RO, Brazil.
Oxid Med Cell Longev. 2018 Jul 5;2018:5456928. doi: 10.1155/2018/5456928. eCollection 2018.
Mancozeb (MZ), a manganese- and zinc-containing ethylene-bis-dithiocarbamate, is a broad-spectrum fungicide. Harmful effects of this fungicide have been reported in nontarget organisms via a not fully understood mechanism. has provided remarkable contributions for toxicological studies. This work was aimed at evaluating the biochemical targets and implication of oxidative stress in MZ-mediated toxicity in drosophilas. Exposure of flies for fifteen days to MZ at 5 and 10 mg/mL through the diet impaired locomotor performance and induced fly mortality. In parallel, it caused lipid peroxidation and reactive oxygen species (ROS) formation and Mn overload. MZ inhibited superoxide dismutase and inducted catalase and glutathione S-transferase activities. Nitric oxide and reduced glutathione levels were significantly decreased by MZ. Heat shock proteins (HSP70 and HSP83) and Nrf2 mRNA levels were significantly augmented in MZ-exposed flies. Our study reinforced the use of as a reliable model for the study of biochemical targets of pesticides, and based on our data, MZ induced oxidative damage and Mn accumulation in a concentration-dependent manner. An adaptative cellular state was inducted by the lower concentration of pesticide, possibly contributing to the slighter damage observed.
代森锰锌（MZ）是一种含有锰和锌的乙撑双二硫代氨基甲酸盐，是一种广谱杀菌剂。通过一种尚未完全了解的机制，已在非靶标生物中报道了这种杀菌剂的有害影响。果蝇为毒理学研究提供了显著的贡献。这项工作旨在评估生化靶标和氧化应激在代森锰锌介导的果蝇毒性中的作用。通过饮食将 5 和 10 mg/mL 的代森锰锌暴露于果蝇 15 天，会损害其运动表现并诱导其死亡。同时，它会引起脂质过氧化和活性氧（ROS）形成以及锰过载。代森锰锌抑制超氧化物歧化酶并诱导过氧化氢酶和谷胱甘肽 S-转移酶的活性。代森锰锌会显著降低一氧化氮和还原型谷胱甘肽的水平。热休克蛋白（HSP70 和 HSP83）和 Nrf2 mRNA 水平在暴露于代森锰锌的果蝇中显著增加。我们的研究强化了使用 作为研究农药生化靶标的可靠模型，并且根据我们的数据，代森锰锌以浓度依赖的方式诱导氧化损伤和锰积累。较低浓度的农药可能会诱导适应性细胞状态，从而导致观察到的损伤较小。
