Levi-Schaffer F, Segal V, Mekori Y A, Claman H N, Hammel I
Department of Pharmacology, Hadassah Medical School, Hebrew University of Jerusalem, Israel.
Immunol Lett. 1991 Jan;27(1):13-8. doi: 10.1016/0165-2478(91)90237-5.
Chronic graft-vs.-host disease (cGVHD) includes a syndrome of inflammatory and fibrotic changes in some respects resembling scleroderma. In the present study we have quantitated the number of peritoneal mast cells (MC) in mice with cGVHD induced across minor histocompatibility barriers. MC were evaluated by staining with toluidine blue. The number of MC decreased significantly (by 25%) at the onset of the cGVHD fibrosis (day 12). Around day 35, MC were virtually undetectable, and started to reappear on day 130. Upon clinical recovery (day 200) a dramatic increase in MC numbers was found (about 8-fold). In addition, we evaluated by electron microscopy the morphology of peritoneal MC, obtained from normal mice and rats, that had been co-cultured on 3T3 fibroblast monolayer in the presence of splenocyte supernatants from mice with cGVHD or from control mice. After 6-8 days of continuous incubation with the cGVHD splenocyte supernatant, MC appeared to be activated, since they displayed an array of heterogenous granules. Few of the granules were dense; many were swollen and pale. Rare granule extrusion was evident. This would indicate that MC underwent a slow activation process due to a factor(s) present in the cGVHD supernatant, different from the classical acute anaphylactic activation.
慢性移植物抗宿主病（cGVHD）包括一种炎症和纤维化改变的综合征，在某些方面类似于硬皮病。在本研究中，我们对通过次要组织相容性屏障诱导产生cGVHD的小鼠腹腔肥大细胞（MC）数量进行了定量。通过甲苯胺蓝染色评估MC。在cGVHD纤维化开始时（第12天），MC数量显著减少（减少25%）。在第35天左右，几乎检测不到MC，而在第130天开始重新出现。临床恢复时（第200天），发现MC数量急剧增加（约8倍）。此外，我们通过电子显微镜评估了从正常小鼠和大鼠获得的腹腔MC的形态，这些MC在存在cGVHD小鼠或对照小鼠脾细胞上清液的情况下，在3T3成纤维细胞单层上共同培养。在用cGVHD脾细胞上清液连续孵育6 - 8天后，MC似乎被激活，因为它们呈现出一系列异质性颗粒。很少有颗粒致密；许多颗粒肿胀且颜色浅。明显可见罕见的颗粒外排。这表明MC由于cGVHD上清液中存在的一种或多种因子而经历了一个缓慢的激活过程，这与经典的急性过敏激活不同。
