Adams Stephen R
Cold Spring Harb Protoc. 2010 Mar;2010(3):pdb.top70. doi: 10.1101/pdb.top70.
In the last two decades, imaging of fluorescent indicators specific for Ca(2+) has revealed its often spectacular spatial dynamics, such as rhythmic oscillations or standing gradients, within single groups or individual cells, in unprecedented detail. This short review describes how the more widely used indicators work. The currently used Ca(2+) indicators have a modular design consisting of a metal-binding site (or sensor) coupled in some way to a fluorescent dye. Combining different sensors with different dyes results in numerous indicators suited to a wide range of experiments and equipment.
在过去二十年中，针对Ca(2+)的荧光指示剂成像以前所未有的细节揭示了其在单个细胞群或单个细胞内通常惊人的空间动态，例如节律性振荡或稳定梯度。这篇简短综述描述了更广泛使用的指示剂的工作原理。目前使用的Ca(2+)指示剂具有模块化设计，由以某种方式与荧光染料偶联的金属结合位点（或传感器）组成。将不同的传感器与不同的染料相结合，可产生适用于广泛实验和设备的众多指示剂。
