Mäkelä Anna R, Ernst Wolfgang, Grabherr Reingard, Oker-Blom Christian
Cold Spring Harb Protoc. 2010 Mar;2010(3):pdb.top72. doi: 10.1101/pdb.top72.
The baculovirus expression vector system has been used extensively to produce numerous proteins originating from both prokaryotic and eukaryotic sources. In addition to easy cloning techniques and abundant viral propagation, the system's insect cell environment provides eukaryotic post-translational modification machinery. The recently established eukaryotic molecular biology tool, the baculovirus display vector system (BDVS), allows the combination of genotype with phenotype, enabling presentation of foreign peptides or even complex proteins on the baculoviral envelope or capsid. This strategy is important because it can be used to enhance viral binding and entry to mammalian cells as well as to produce antibodies against the displayed antigen. In addition, the technology should enable modifications of intracellular behavior, that is, trafficking of recombinant "nanoparticles," a highly relevant feature for studies of targeted gene or protein delivery. This article discusses the design and potential uses of insect-derived baculoviral display vectors.
杆状病毒表达载体系统已被广泛用于生产源自原核和真核生物的多种蛋白质。除了简便的克隆技术和丰富的病毒繁殖能力外，该系统的昆虫细胞环境还提供真核生物的翻译后修饰机制。最近建立的真核分子生物学工具——杆状病毒展示载体系统（BDVS），能够将基因型与表型相结合，使外源肽甚至复杂蛋白质呈现在杆状病毒包膜或衣壳上。这一策略很重要，因为它可用于增强病毒与哺乳动物细胞的结合及进入，还能生产针对所展示抗原的抗体。此外，该技术应能实现细胞内行为的修饰，即重组“纳米颗粒”的运输，这对于靶向基因或蛋白质递送研究来说是一个高度相关的特性。本文讨论了昆虫源杆状病毒展示载体的设计及潜在用途。
