van Muijen G N, Jansen K F, Cornelissen I M, Smeets D F, Beck J L, Ruiter D J
Department of Pathology, University Hospital Nijmegen, The Netherlands.
Int J Cancer. 1991 Apr 22;48(1):85-91. doi: 10.1002/ijc.2910480116.
To select human melanoma cells that are highly tumorigenic and metastatic in nude mice we have implanted fragments of a fresh human melanoma metastasis subcutaneously (s.c.) into a nude mouse. After 3 passages in nude mice, part of the xenograft was cultured and a new melanoma cell line, MV3, was established. After intravenous (i.v.) inoculation of 2 x 10(6) MV3 cells, 95% of the nude mice (n = 20) developed lung colonies within 6 weeks. S.c. inoculation of 2 x 10(6) MV3 cells resulted in 95% tumor take, while 90% of the mice (n = 20) showed spontaneous metastases in the lungs within 7 weeks. Histological and immunohistological features of the original tumor of the patient were largely retained in the tumors of the mice and in the cell line in vitro. As shown by Alcian blue staining, MV3 cells contain large quantities of glycosaminoglycans (GAGs) and/or proteoglycanes (PGs), both in vivo and in vitro. The cells showed a marked expression of transferrin receptor, ICAM-1, EGF-receptor, and VLA-2 integrin. As only few human melanoma cell lines are available that frequently show metastasis in nude mice, the highly metastatic MV3 cell line represents a useful tool for studying the expression and regulation of molecules on human melanoma cells involved in the process of metastasis.
为了筛选出在裸鼠中具有高致瘤性和转移性的人黑色素瘤细胞，我们将新鲜的人黑色素瘤转移灶碎片皮下植入裸鼠体内。在裸鼠体内传代3次后，取部分异种移植瘤进行培养，建立了一种新的黑色素瘤细胞系MV3。静脉注射2×10⁶个MV3细胞后，95%的裸鼠（n = 20）在6周内出现肺部转移灶。皮下接种2×10⁶个MV3细胞，肿瘤接种成功率为95%，而90%的小鼠（n = 20）在7周内出现肺部自发性转移。患者原发肿瘤的组织学和免疫组织学特征在小鼠肿瘤及体外细胞系中基本得以保留。阿尔辛蓝染色显示，MV3细胞在体内和体外均含有大量糖胺聚糖（GAGs）和/或蛋白聚糖（PGs）。这些细胞转铁蛋白受体、细胞间黏附分子-1（ICAM-1）、表皮生长因子受体（EGF-受体）和VLA-2整合素表达明显。由于在裸鼠中经常发生转移的人黑色素瘤细胞系很少，高度转移性的MV3细胞系为研究参与转移过程的人黑色素瘤细胞上分子的表达和调控提供了一个有用的工具。
