Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Nanotechnology. 2010 Mar 26;21(12):125203. doi: 10.1088/0957-4484/21/12/125203. Epub 2010 Mar 2.
Using a one-dimensional grating surface photonic crystal (PC), we experimentally demonstrate that the detection of fluorescent molecules on a PC surface can be substantially magnified through the combined effects of resonance-enhanced excitation of the fluorescent dye, resonance-enhanced extraction of the fluorescence emission and a dielectric nanorod surface coating increasing the surface area available for fluorophore-PC interaction. Enhanced excitation is obtained by engineering a high-Q TM resonant mode to efficiently couple with an incident TM-polarized lambda = 633 nm laser for exciting Cyanine-5 (Cy5). Enhanced extraction results from a low-Q TE resonance designed to spectrally overlap the Cy5 emission spectrum for channeling TE-polarized emission towards the detection instrument. The entire PC surface is coated with a porous film of TiO(2) nanorods that allows more fluorophores to penetrate into the region of enhanced near-electric fields. Experimental results reveal a 588-fold enhancement in fluorescence intensity relative to an unpatterned glass surface.
我们使用一维光栅表面光子晶体(PC),通过荧光染料的共振增强激发、荧光发射的共振增强提取以及增加用于荧光染料-PC 相互作用的表面积的介电纳米棒表面涂层的联合作用,实验证明了在 PC 表面上检测荧光分子的能力可以得到极大增强。通过设计一个高 Q 值的 TM 共振模式来有效地与入射的 TM 偏振 λ=633nm 激光耦合,从而对 Cy5 进行激发,获得了增强的激发。通过设计一个低 Q 值的 TE 共振,使其与 Cy5 发射光谱光谱重叠,将 TE 偏振发射引导到检测仪器,实现了增强的提取。整个 PC 表面都涂有一层 TiO(2)纳米棒的多孔薄膜,允许更多的荧光染料渗透到增强的近电场区域。实验结果显示,与无图案的玻璃表面相比,荧光强度增强了 588 倍。