Boisson-Vidal C, Jozefonvicz J, Brash J L
Laboratoire de Recherches sur les Macromolécules, C.S.P., C.N.R.S., Villetaneuse, France.
J Biomed Mater Res. 1991 Jan;25(1):67-84. doi: 10.1002/jbm.820250106.
Investigations are reported on the composition of protein layers adsorbed from plasma to various modified polystyrene resins. As well as polystyrene itself, polystyrene bearing sulfonate groups in the benzene rings, and polystyrene sulfonate in which the sulfonate groups were converted to amino acid sulfamide, were investigated. Some of these resins were shown in previous work to have anticoagulant properties. To study the adsorption of proteins from plasma, the resins were exposed to citrate anticoagulated human plasma for 3 h. Adsorbed proteins were then eluted sequentially by 1M Tris buffer and 4% SDS solution, and examined by SDS-PAGE. The gel patterns were similar on all resins except polystyrene. From the MWs of the gel bands, the major protein component appeared to be fibrinogen. Smaller amounts of plasminogen, transferrin, albumin, and IgG were also present. In addition, Ouchterlony immunoassay of the eluates from one resin gave positive identification of complement C3, fibronectin, IgG, and IgM. Many other minor gel bands remain unidentified. A consistent finding for all resins was the presence of plasmin-type fibrinogen degradation products though the amounts varied with resin type. It is concluded from this (and from experiments showing FDP formation when fibrinogen was absorbed to the resins, from buffer containing a trace of plasminogen) that the functional groups in these materials promote the adsorption of plasminogen and its activation to a plasmin-like molecule. It appears from the substantial quantities of fibrinogen adsorbed to these materials after 3 h exposure to plasma that the Vroman effect (giving transient adsorption of fibrinogen) is not operative on these materials. It is hypothesized that specific interactions occur between fibrinogen and sulfonate groups.
本文报道了关于从血浆吸附到各种改性聚苯乙烯树脂上的蛋白质层组成的研究。除了聚苯乙烯本身外，还研究了苯环上带有磺酸基团的聚苯乙烯以及磺酸基团已转化为氨基酸磺酰胺的聚苯乙烯磺酸盐。在先前的研究中已表明其中一些树脂具有抗凝血特性。为了研究血浆中蛋白质的吸附情况，将这些树脂暴露于柠檬酸盐抗凝的人血浆中3小时。然后依次用1M Tris缓冲液和4% SDS溶液洗脱吸附的蛋白质，并通过SDS-PAGE进行检测。除聚苯乙烯外，所有树脂上的凝胶图谱都相似。从凝胶条带的分子量来看，主要蛋白质成分似乎是纤维蛋白原。还存在少量的纤溶酶原、转铁蛋白、白蛋白和IgG。此外，对一种树脂洗脱液的双向免疫扩散试验阳性鉴定出补体C3、纤连蛋白、IgG和IgM。许多其他较小的凝胶条带仍未鉴定出来。所有树脂的一个一致发现是存在纤溶酶型纤维蛋白原降解产物，尽管其含量因树脂类型而异。由此得出结论（以及从纤维蛋白原吸附到树脂上时在含有微量纤溶酶原的缓冲液中显示FDP形成的实验得出的结论），这些材料中的官能团促进纤溶酶原的吸附及其激活为类似纤溶酶的分子。从在血浆中暴露3小时后吸附到这些材料上的大量纤维蛋白原来看，Vroman效应（导致纤维蛋白原的瞬时吸附）在这些材料上不起作用。据推测，纤维蛋白原与磺酸基团之间发生了特异性相互作用。
