噬菌体展示技术的进展：技术的演变及其应用。
Progress in phage display: evolution of the technique and its application.
机构信息
Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000 Ljubljana, Slovenia.
出版信息
Cell Mol Life Sci. 2010 Mar;67(5):749-67. doi: 10.1007/s00018-009-0192-2.
Abstract
Phage display, the presentation of (poly)peptides as fusions to capsid proteins on the surface of bacterial viruses, celebrates its 25th birthday in 2010. The technique, coupled with in vitro selection, enables rapid identification and optimization of proteins based on their structural or functional properties. In the last two decades, it has advanced tremendously and has become widely accepted by the scientific community. This by no means exhaustive review aims to inform the reader of the key modifications in phage display. Novel display formats, innovative library designs and screening strategies are discussed. I will also briefly review some recent uses of the technology to illustrate its incredible versatility.
摘要
噬菌体展示技术是将（多）肽融合到细菌病毒衣壳蛋白表面的一种展示技术，于 2010 年迎来了它的 25 岁生日。该技术与体外选择相结合，可以快速鉴定和优化基于结构或功能特性的蛋白质。在过去的二十年中，它取得了巨大的进步，并被科学界广泛接受。本文旨在向读者介绍噬菌体展示技术的关键改进，并不详尽。本文讨论了新型展示格式、创新文库设计和筛选策略。我还将简要回顾该技术的一些最新用途，以说明其令人难以置信的多功能性。
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