[Involvement of endoplasmic reticulum stress in development of fatty liver fibrosis induced by methionine-choline-deficient diet in rats].
作者信息
Mu Yong-ping, Kwada Norifumi, Ogawa Tomohiro, Xi Xiu-hong, Chen Xiao-rong
机构信息
Shanghai Public Health Clinical Centre, Shanghai 201508, China.
出版信息
Zhonghua Gan Zang Bing Za Zhi. 2010 Feb;18(2):124-30. doi: 10.3760/cma.j.issn.1007-3418.2010.02.011.
OBJECTIVE
To study role of endoplasmic reticulum stress in the development of fatty liver fibrosis induced by methionine-choline-deficient diet in rats.
METHODS
Non-alcoholic steatohepatitis was induced by 10 weeks- methionine-choline-deficient diet (MCDD), Markers of endoplasmic reticulum stress were determined by immunoblotting and real-time PCR.
RESULTS
The number of apoptotic hepatocytes, The expression levels of endoplasmic reticulum stress markers were increased significantly in MCDD group compared to control group (probability value less than 0.05 or probability value less than 0.01), while ratio of hepatocyte proliferation/apoptosis was decreased in MCDD group (probability value less than 0.01). The number of hepatocytes apoptosis, and the expression levels of endoplasmic reticulum stress markers were decreased significantly 2 weeks after the feeding with normal diet in MCDD group (probability value less than 0.05 or probability value less than 0.01).
CONCLUSION
MCDD induces endoplasmic reticulum stress and fibrosis in rats.
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