Li Rong-kuan, Tang Jian-wu, Zhang Jun, Wang Shao-qing, Wang Mei, Wang Bo, Zhang Yu-hong
Department of Gastroenterology and Hepatology, the Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China.
Zhonghua Gan Zang Bing Za Zhi. 2010 Feb;18(2):131-5. doi: 10.3760/cma.j.issn.1007-3418.2010.02.012.
To study the effects of silencing CLIC1 gene expression on the proliferation and invasion of Hca-F cells.
The mouse CLIC1 cDNA sequence was retrieved from NCBI. Three shRNA sequences were designed and cloned into pGPU6/GFP/Neo plasmids. The plasmids were transfected into Hca-F cells with Lipofectamine 2000. Cell Counting-8 (CCK-8) kit and transwell chamber were used to study the effects of CLIC1 on the proliferation and invasion of Hca-F cells.
The pGPU6/GFP/Neo-shRNA-3 plasmid effectively repressed the expression of CLIC1 mRNA. Inhibition of CLIC1 gene expression led to decreased cell proliferation and reduced invasion.
CLIC1 is essential for the proliferation and invasion of Hca-F cells.
研究沉默CLIC1基因表达对Hca-F细胞增殖和侵袭的影响。
从NCBI检索小鼠CLIC1 cDNA序列。设计三条shRNA序列并克隆到pGPU6/GFP/Neo质粒中。用Lipofectamine 2000将质粒转染到Hca-F细胞中。使用细胞计数-8（CCK-8）试剂盒和Transwell小室研究CLIC1对Hca-F细胞增殖和侵袭的影响。
pGPU6/GFP/Neo-shRNA-3质粒有效抑制CLIC1 mRNA的表达。CLIC1基因表达的抑制导致细胞增殖减少和侵袭能力降低。
CLIC1对Hca-F细胞的增殖和侵袭至关重要。
