六种来自伊朗的鼠尾草属植物的体外抗氧化活性和神经保护作用研究：一项比较研究。

In vitro antioxidant activities and an investigation of neuroprotection by six Salvia species from Iran: a comparative study.

机构信息

Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

Food Chem Toxicol. 2010 May;48(5):1341-9. doi: 10.1016/j.fct.2010.02.035. Epub 2010 Mar 1.

DOI:10.1016/j.fct.2010.02.035

PMID:20197079

Abstract

Methanolic extracts of six species of Salvia (S. hydrangea, S. lachnocalyx, S. macilenta, S. multicalis, S. sclarea and S. xanthocheila) were analyzed for their antioxidant properties, ability to prevent DNA damage by free radicals, and neuroprotective effects. Several biochemical assays were used to evaluate their antioxidant properties: DPPH(), FRAP, beta-carotene bleaching and TEAC assays. The amounts of phenolics and flavonoids were also determined. Comparison study of Salvia species showed that extracts from S. hydrangea and S. macilenta are strong antioxidants and that from S. lachnocalyx is a weak one. Furthermore, extracts from all of these species can at high concentrations (50mug/ml) inhibit DNA damage by free radicals. Furthermore, these species not only showed no cytotoxic effects in nerve growth factor (NGF)-differentiated PC12 cells, they also protected them against H(2)O(2)-induced cell death. Thus these plants may be candidates for treating neurodegenerative diseases.

摘要

六种鼠尾草属植物（穗花鼠尾草、宽苞鼠尾草、毛地黄鼠尾草、多茎鼠尾草、香紫苏和黄花鼠尾草）的甲醇提取物被分析其抗氧化性能、预防自由基引起的 DNA 损伤的能力和神经保护作用。几种生化测定法被用于评估其抗氧化性能：DPPH（）、FRAP、β-胡萝卜素漂白和 TEAC 测定。多酚和类黄酮的含量也被测定。鼠尾草属植物的比较研究表明，穗花鼠尾草和毛地黄鼠尾草的提取物具有很强的抗氧化能力，而宽苞鼠尾草的提取物则较弱。此外，所有这些物种的提取物在高浓度（50μg/ml）下可以抑制自由基引起的 DNA 损伤。此外，这些物种不仅在神经生长因子（NGF）分化的 PC12 细胞中没有细胞毒性作用，而且还可以防止它们受到 H（2）O（2）诱导的细胞死亡。因此，这些植物可能是治疗神经退行性疾病的候选药物。