Siqueira Ionara Rodrigues, Vanzella Cláudia, Lovatel Gisele Agustini, Bertoldi Karine, Spindler Christiano, Moysés Felipe Dos Santos, Vizuete Adriana, Poser Gilsane Lino von, Netto Carlos Alexandre
Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, RS, Brazil.
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, RS, Brazil.
Nutrients. 2025 Jul 19;17(14):2368. doi: 10.3390/nu17142368.
: There is evidence concerning herbal medicines and plant-based compounds, including Lamiaceae species, as putative senolytic agents; however, there are only a few reports on properties using rat models. The aim of this study was to investigate the neuroprotective effects and potential modes of action of L. using ex vivo and in vivo assays to assess the effects of OAEE on hippocampal tissue from young adult and late middle-aged Wistar rats, with a focus on oxidative stress, cholinesterase activity, and neuroinflammatory markers. : ethanol extract (OAEE) was incubated with hippocampal slices of young adult and late middle-aged male Wistar rats exposed to HO; an acute treatment with OAEE was evaluated in aversive memory performance and neurochemical parameters, such as hippocampal cellular oxidative state, and anticholinesterase activity, and a diet supplementation of OAEE were evaluated on several hippocampal biochemical parameters, such as oxidative state, anticholinesterase activity, and neuroinflammatory parameters in young adult and late middle-aged male rats. : OAEE reversed the HO-induced impaired cellular viability in hippocampal slices from young adult rats, as well as protected hippocampal slices against HO-induced damage in both young adult and late middle-aged Wistar rats, indicating its neuroprotective action. Chronic dietary OAEE supplementation reduced aging-induced increases in reactive species and lipid peroxidation levels in the hippocampus. Indeed, this supplementation reduced the TNF-α content in hippocampus from both ages, and IL-1β levels in young adult rats. : The antioxidant actions of OAEE here observed, preventing the lipoperoxidation, as well as its anti-neuroinflammatory effect, might be related to neuroprotective effect. Our findings add evidence to support the idea of the potential use of as a nutraceutical or functional food in the aging process.
有证据表明，包括唇形科植物在内的草药和植物性化合物可能是溶酶体清除剂；然而，关于使用大鼠模型的特性的报道却很少。本研究的目的是通过体外和体内试验，研究迷迭香叶乙醇提取物（OAEE）对年轻成年和中老年Wistar大鼠海马组织的神经保护作用及其潜在作用模式，重点关注氧化应激、胆碱酯酶活性和神经炎症标志物。将OAEE与暴露于过氧化氢（HO）的年轻成年和中老年雄性Wistar大鼠的海马切片孵育；在厌恶记忆表现和神经化学参数（如海马细胞氧化状态和抗胆碱酯酶活性）方面评估OAEE的急性治疗效果，并在年轻成年和中老年雄性大鼠中评估OAEE饮食补充对几种海马生化参数（如氧化状态、抗胆碱酯酶活性和神经炎症参数）的影响。OAEE逆转了HO诱导的年轻成年大鼠海马切片中细胞活力受损的情况，同时保护年轻成年和中老年Wistar大鼠的海马切片免受HO诱导的损伤，表明其具有神经保护作用。长期饮食补充OAEE可降低衰老诱导的海马中活性物质增加和脂质过氧化水平。事实上，这种补充降低了两个年龄段海马中的肿瘤坏死因子-α（TNF-α）含量以及年轻成年大鼠中的白细胞介素-1β（IL-1β）水平。此处观察到的OAEE的抗氧化作用，即防止脂质过氧化，以及其抗神经炎症作用，可能与神经保护作用有关。我们的数据为支持迷迭香叶在衰老过程中作为营养保健品或功能性食品的潜在用途这一观点增添了证据。
