Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan.
Colloids Surf B Biointerfaces. 2010 Jun 1;77(2):270-8. doi: 10.1016/j.colsurfb.2010.02.009. Epub 2010 Feb 11.
Polyelectrolyte multilayer (PEM) films have been recently applied to surface modification of biomaterials. Cellular interactions with PEM films consisted of weak polyelectrolytes are greatly affected by the conditions of polyelectrolyte deposition, such as pH of polyelectrolyte solution. Previous studies indicated that the adhesion of several types of mammalian cells to PAH/PAA multilayer films was hindered by low pH and high layer numbers. The objective of this study is to evaluate whether the hemocompatibility of polysulfone can be modulated by deposition of poly(allylamine hydrochloride) (PAH)/poly(acrylic acid) (PAA) multilayer films. PAH/PAA multilayer films with different layer numbers were assembled onto polysulfone at either pH 2.0 or pH 6.5. The number of platelet adhesion and the morphology of adherent platelets were determined to evaluate hemocompatibility of modified substrates. Compared to non-treat polysulfone, the PEM films developed at pH 2.0 decreased platelet adhesion, while those built at pH 6.5 enhanced platelet deposition. Platelet adhesion was found positively correlated to polyclonal antibodies binding to surface-bound fibrinogen. The extent of platelet spreading was increased with layer numbers of PEM films, suggesting that the adherent platelets on thick PEM films were prone to activation. In conclusion, PAH/PAA films with few layers developed at pH 2.0 possessed better hemocompatibility compared to other substrates.
聚电解质多层（PEM）薄膜最近已被应用于生物材料的表面改性。细胞与由弱聚电解质组成的 PEM 薄膜的相互作用受聚电解质沉积条件的极大影响，例如聚电解质溶液的 pH 值。先前的研究表明，几种类型的哺乳动物细胞对 PAH/PAA 多层膜的黏附受到低 pH 值和高层数的阻碍。本研究的目的是评估聚砜的血液相容性是否可以通过沉积聚（盐酸烯丙胺）（PAH）/聚（丙烯酸）（PAA）多层膜来调节。在 pH 值为 2.0 或 6.5 下，将具有不同层数的 PAH/PAA 多层膜组装到聚砜上。通过测定血小板黏附的数量和黏附血小板的形态来评估改性基底的血液相容性。与未经处理的聚砜相比，在 pH 值为 2.0 下制备的 PEM 薄膜减少了血小板黏附，而在 pH 值为 6.5 下制备的 PEM 薄膜则增强了血小板沉积。血小板黏附与表面结合纤维蛋白原的多克隆抗体结合呈正相关。血小板铺展的程度随 PEM 膜层数的增加而增加，表明厚 PEM 膜上的黏附血小板容易被激活。总之，与其他基底相比，在 pH 值为 2.0 下制备的具有较少层数的 PAH/PAA 膜具有更好的血液相容性。
