Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
Biomacromolecules. 2011 Dec 12;12(12):4348-56. doi: 10.1021/bm2013185. Epub 2011 Nov 21.
Zwitterionic sulfobetaine methacrylate (SBMA) polymers were known to possess excellent antifouling properties due to high hydration capacity and neutral charge surface. In this study, copolymers of SBMA and acrylic acid (AA) with a variety of compositions were synthesized and were immobilized onto polymeric substrates with layer-by-layer polyelectrolyte films via electrostatic interaction. The amounts of platelet adhesion and fibrinogen adsorption were determined to evaluate hemocompatibility of poly(SBMA-co-AA)-modified substrates. Among various deposition conditions by modulating SBMA ratio in the copolymers and pH of the deposition solution, poly(SBMA(56)-co-AA(44)) deposited at pH 3.0 possessed the best hemocompatibility. This work demonstrated that poly(SBMA-co-AA) copolymers adsorbed on polyelectrolyte-base films via electrostatic interaction improve hemocompatibility effectively and are applicable for various substrates including TCPS, PU, and PDMS. Furthermore, poly(SBMA-co-AA)-coated substrate possesses great durability under rigorous conditions. The preliminary hemocompatibility tests regarding platelet adhesion, fibrinogen adsorption, and plasma coagulation suggest the potential of this technique for the application to blood-contacting biomedical devices.
两性离子磺酸甜菜碱甲基丙烯酸酯 (SBMA) 聚合物由于高水合容量和中性电荷表面而具有优异的抗污性能。在这项研究中，合成了 SBMA 和丙烯酸 (AA) 的各种组成的共聚物，并通过静电相互作用将其固定在带有层层聚电解质膜的聚合物基底上。通过测定血小板黏附和纤维蛋白原吸附的量来评估聚 (SBMA-co-AA)-修饰基底的血液相容性。在通过调节共聚物中的 SBMA 比例和沉积溶液的 pH 值来改变各种沉积条件下，在 pH 3.0 下沉积的聚 (SBMA(56)-co-AA(44)) 具有最佳的血液相容性。这项工作表明，通过静电相互作用吸附在聚电解质基底上的聚 (SBMA-co-AA) 共聚物可有效提高血液相容性，适用于包括 TCPS、PU 和 PDMS 在内的各种基底。此外，聚 (SBMA-co-AA) 涂层基底在严格条件下具有出色的耐用性。关于血小板黏附、纤维蛋白原吸附和血浆凝固的初步血液相容性测试表明，该技术在血液接触式生物医学设备中的应用具有潜力。
