[子痫前期患者胎盘床HIF-1α蛋白表达及母体外周血中vWF浓度的变化]

[Change of HIF-1alpha protein expression in the placenta bed and concentration of vWF in maternal peripheral blood of pre-eclampsia].

作者信息

Peng Mei, Ding Yiling, Yu Ling, Zhou Changju

机构信息

Department of Gynecology and Obstetrics, Second Xiangya Hospital, Central South University, Changsha 410011, China.

出版信息

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2010 Feb;35(2):134-9. doi: 10.3969/j.issn.1672-7347.2010.02.008.

DOI:10.3969/j.issn.1672-7347.2010.02.008

PMID:20197612

Abstract

OBJECTIVE

To investigate the expression of HIF-1alpha protein in the placenta bed and the concentration of von Willrand factor (vWF) in maternal peripheral blood from pre-eclampsia and normal pregnancy, and to determine the effect of HIF-1alpha and vWF on the pathogenesis of pre-eclampsia.

METHODS

Forty pre-eclampsia patients (20 mild and 20 severe) were recruited as 2 study groups, and another 20 normal pregnant women were served as a normal group.Western blot was used to detect the expression of HIF-1alpha protein in the placenta bed. ELISA was adopted to detect the concentration of vWF in the maternal peripheral blood.Correlation between HIF-1alpha protein expression and vWF level was analyzed by Spearman method.

RESULTS

The expression of HIF-1alpha protein in the placenta bed was the highest in the severe pre-eclampsia patients among 3 groups, followed by the mild pre-eclampsia patients and the normal controls. There was significant difference among 3 groups (P<0.001). The concentration of vWF in the maternal peripheral blood was the highest in the severe pre-eclampsia patients, followed by the mild pre-eclampsia patients and the normal controls. There was significant difference among the 3 groups (P<0.05). Positive correlation was found between the expression of HIF-1alpha protein in the placenta bed and the concentration of vWF in the maternal peripheral blood in patients with pre-eclampsia (r1=0.65, P<0.001), and between the concentration of vWF in the maternal peripheral blood and the pathogenetic condition degree of pre-eclampsia (r(2)=0.61,P<0.001).

CONCLUSION

HIF-1alpha in coordination with vWF may play an important role in the pathogenesis of pre-eclampsia.

摘要

目的

研究子痫前期患者胎盘床中低氧诱导因子-1α（HIF-1α）蛋白的表达及母体外周血中血管性血友病因子（vWF）的浓度，并确定HIF-1α和vWF在子痫前期发病机制中的作用。

方法

选取40例子痫前期患者（20例轻度和20例重度）作为2个研究组，另选20例正常孕妇作为正常组。采用蛋白质免疫印迹法检测胎盘床中HIF-1α蛋白的表达。采用酶联免疫吸附测定法检测母体外周血中vWF的浓度。用Spearman法分析HIF-1α蛋白表达与vWF水平的相关性。

结果

重度子痫前期患者胎盘床中HIF-1α蛋白的表达在3组中最高，其次是轻度子痫前期患者和正常对照组。3组间差异有统计学意义（P<0.001）。母体外周血中vWF的浓度在重度子痫前期患者中最高，其次是轻度子痫前期患者和正常对照组。3组间差异有统计学意义（P<0.05）。子痫前期患者胎盘床中HIF-1α蛋白的表达与母体外周血中vWF的浓度呈正相关（r1=0.65，P<0.001），母体外周血中vWF的浓度与子痫前期的病情严重程度呈正相关（r2=0.61，P<0.001）。