Park Hyung-Doo, Park Kyoung Un, Song Junghan, Ki Chang-Seok, Han Kyou Sup, Kim Jin Q
Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Korean J Lab Med. 2010 Feb;30(1):51-7. doi: 10.3343/kjlm.2010.30.1.51.
The Lewis histo-blood group system consists of 2 major antigens-Lea and Leb-and a sialyl Lewis antigen-carbohydrate antigen (CA) 19-9. We investigated the distribution of Lewis genotypes and evaluated the relationship between the Lewis/Secretor genotypes and the serum level of CA 19-9 in a Korean population to identify whether the serum CA 19-9 levels are influenced by the Lewis/Secretor genotypes.
The study included 242 individuals who had no malignancies. Lewis genotyping was performed for the 59T>G, 508G>A and 1067T>A polymorphic sites. The Secretor genotype was determined through analysis of the 357C>T and 385A>T polymorphic sites and the fusion gene. Serum CA 19-9 level was analyzed using an electrochemiluminescence immunoassay.
Individuals carrying the 3 common genotypes-Le/Le, Le/le(59,508), and Le/le(59,1067)-accounted for 95% of the study population. In the Korean population, the allelic frequencies of Le, Le(59), le(59,508), and le(59,1067) were 0.731, 0.010, 0.223, and 0.035, respectively. We found a significant difference in serum CA 19-9 concentrations among the 9 Lewis/Secretor genotype groups (P<0.001). The serum CA 19-9 levels in subjects with genotype groups 1 and 2 (Le/- and se/se) were higher than those with genotype groups 3-6 (Le/- and Se/-; 15.63 vs 6.64 kU/L, P<0.001).
Le/Le, Le/le(59,508), and Le/le(59,1067) are frequent Lewis genotypes in Koreans. Because serum CA 19-9 levels are significantly influenced by the Lewis/Secretor genotypes, caution is suggested when interpreting the serum CA 19-9 levels.
Lewis组织血型系统由两种主要抗原——Lea和Leb——以及一种唾液酸化Lewis抗原——碳水化合物抗原（CA）19-9组成。我们调查了韩国人群中Lewis基因型的分布，并评估了Lewis/分泌型基因型与血清CA 19-9水平之间的关系，以确定血清CA 19-9水平是否受Lewis/分泌型基因型的影响。
该研究纳入了242名无恶性肿瘤的个体。对59T>G、508G>A和1067T>A多态性位点进行Lewis基因分型。通过分析357C>T和385A>T多态性位点以及融合基因来确定分泌型基因型。使用电化学发光免疫分析法分析血清CA 19-9水平。
携带三种常见基因型——Le/Le、Le/le(59,508)和Le/le(59,1067)——的个体占研究人群的95%。在韩国人群中，Le、Le(59)、le(59,508)和le(59,1067)的等位基因频率分别为0.731、0.010、0.223和0.035。我们发现9个Lewis/分泌型基因型组之间的血清CA 19-9浓度存在显著差异（P<0.001）。基因型组1和2（Le/-和se/se）的受试者血清CA 19-9水平高于基因型组3-6（Le/-和Se/-；15.63对6.64 kU/L，P<0.001）。
Le/Le、Le/le(59,508)和Le/le(59,1067)是韩国人中常见的Lewis基因型。由于血清CA 19-9水平受Lewis/分泌型基因型的显著影响，因此在解释血清CA 19-9水平时建议谨慎。
