多焦平面显微镜的三维分辨率测量
3D RESOLUTION MEASURE FOR MULTIFOCAL PLANE MICROSCOPY.
作者信息
Chao Jerry, Ram Sripad, Ward E Sally, Ober Raimund J
机构信息
Department of Electrical Engineering, University of Texas at Dallas, Richardson, TX.
出版信息
Proc IEEE Int Symp Biomed Imaging. 2008;4541252:1339-1342. doi: 10.1109/ISBI.2008.4541252.
The distance separating two biomolecules in close proximity is an important determinant of the nature of their interaction. While much focus has been given to resolving distances in 2D, the 3D cell in which biological interactions occur necessitates the evaluation of resolution in 3D. Recently, we introduced an information-theoretic 3D resolution measure which predicts that the resolution of an optical microscope is unlimited, and that it improves as more photons are detected from the imaged molecules. Here, we investigate the 3D resolution measure for a multifocal plane microscope. Used for the simultaneous imaging of distinct focal planes within a specimen, multifocal plane microscopy has important applications in the tracking of microscopic objects in 3D. By comparing their 3D resolution measures, we determine the circumstances under which a two-plane microscope setup offers better resolvability than a comparable conventional microscope.
紧密相邻的两个生物分子之间的距离是其相互作用性质的重要决定因素。虽然人们一直非常关注二维距离的解析，但生物相互作用发生的三维细胞需要对三维分辨率进行评估。最近，我们引入了一种信息论三维分辨率度量方法，该方法预测光学显微镜的分辨率是无限的，并且随着从成像分子中检测到的光子增多而提高。在此，我们研究了多焦平面显微镜的三维分辨率度量方法。多焦平面显微镜用于同时成像标本内不同的焦平面，在三维微观物体跟踪方面有重要应用。通过比较它们的三维分辨率度量方法，我们确定了双平面显微镜设置比同类传统显微镜具有更好分辨率的情况。
Zotero 插件