Ponnala Lalit
Computational Biology Service Unit, Cornell University, Ithaca, NY 14853, USA.
Bioinformation. 2009 Sep 5;4(2):63-5. doi: 10.6026/97320630004063.
Long stretches of "rare" codons are known to severely inhibit the efficiency of translation. Understanding the distribution of such rare codons is of critical importance in improving the efficiency of heterologous gene expression systems. Accurate estimates of codon usage take the abundance of each protein into consideration. In this paper, we analyze the correlation between approximate measures of codon usage and the availability of tRNA at various growth rates in E coli. We show that the computationally derived estimates of tRNA isoacceptor concentration enable the finding of poorly translated codons.
已知长片段的“稀有”密码子会严重抑制翻译效率。了解此类稀有密码子的分布对于提高异源基因表达系统的效率至关重要。准确估计密码子使用情况会考虑每种蛋白质的丰度。在本文中，我们分析了大肠杆菌中不同生长速率下密码子使用的近似度量与tRNA可用性之间的相关性。我们表明，通过计算得出的tRNA同工受体浓度估计值能够找出翻译效果不佳的密码子。
