Barbieri R, Silvestri A
Dipartimento di Chimica Inorganica, Universita di Palermo, Italy.
J Inorg Biochem. 1991 Jan;41(1):31-5. doi: 10.1016/0162-0134(91)85006-3.
The reaction of aqueous native DNA (calf thymus) with the solvated organotin(IV) species [(CH3)2SnCl2(C2H5OH)n], as well as with [(CH3)2Sn(OH)(H2O)n]+ and (CH3)2Sn(OH)2 (i.e., the hydrolysis products of aqueous (CH3)2SnCl2 at pH approximately 5 and pH approximately 7.4 respectively), was investigated by 119Sn Mössbauer spectroscopy. The addition of [(CH3)2SnCl2(C2H5OH)n] to DNA yielded a solid product, possibly (CH3)2Sn(DNA phosphodiester)2, where the environment of the tin atom is trans-octahedral with linear CSnC skeleton, and the equatorial atoms may consist of oxygen or nitrogen from water as well as from the nucleic acid constituents. No interaction with DNA apparently takes place due to hydrolyzed dimethyltin(IV) species, which occur in aqueous phases at approximate physiological pH values. The reaction pathway is then assumed to require weakly solvated, easily dissociable species such as [(CH3)2SnCl2(C2H5OH)n], which would imply in vivo reactivity of cellular DNA with organotins from hydrophobic sites.
采用¹¹⁹Sn穆斯堡尔光谱研究了天然水相DNA（小牛胸腺DNA）与溶剂化有机锡（IV）物种[(CH₃)₂SnCl₂(C₂H₅OH)ₙ]以及[(CH₃)₂Sn(OH)(H₂O)ₙ]⁺和(CH₃)₂Sn(OH)₂（即分别在pH约为5和pH约为7.4的水相中(CH₃)₂SnCl₂的水解产物）的反应。向DNA中添加[(CH₃)₂SnCl₂(C₂H₅OH)ₙ]会产生一种固体产物，可能是(CH₃)₂Sn(DNA磷酸二酯)₂，其中锡原子的环境为具有线性CSnC骨架的反式八面体，赤道原子可能由来自水以及核酸成分的氧或氮组成。在接近生理pH值的水相中出现的水解二甲基锡（IV）物种显然不会与DNA发生相互作用。因此推测反应途径需要弱溶剂化、易于解离的物种，如[(CH₃)₂SnCl₂(C₂H₅OH)ₙ]，这意味着细胞DNA在体内会与来自疏水部位的有机锡发生反应。
