Sizova O I, Kochetkov V V, Boronin A M
Prikl Biokhim Mikrobiol. 2010 Jan-Feb;46(1):45-50.
Rhizosphere strains of P. aureofaciens BS1393(pBS216, pKS1) and P. chlororaphis PCL1391(pBS216, pKS1), exhibiting the ability to stimulate the growth of plants and protect them from phytopathogens, have been obtained. In these strains, plasmid pBS216 ensures naphthalene degradation and plasmid pKS1 confers resistance to arsenic. In the presence of arsenic and naphthalene, the number of living cells and the growth rate of the arsenic-resistant strains were higher than those of the arsenic-sensitive strains BS1393(pBS216) and PCL1391(pBS216). During the cultivation of the resistant strains, arsenic had no inhibitory effect on the activity of the key enzymes of naphthalene biodegradation, except for catechol-2,3-dioxygenase. In a model system containing plant-microbial associations, strains BS1393(pBS216, pKS1) and PCL1391(pBS216, pKS1) degraded as much as 97% of added naphthalene in the presence of arsenic.
已获得金黄色荧光假单胞菌BS1393(pBS216, pKS1)和绿色产色链霉菌PCL1391(pBS216, pKS1)的根际菌株，这些菌株具有促进植物生长并保护其免受植物病原体侵害的能力。在这些菌株中，质粒pBS216确保萘的降解，质粒pKS1赋予对砷的抗性。在存在砷和萘的情况下，抗砷菌株的活细胞数量和生长速率高于砷敏感菌株BS1393(pBS216)和PCL1391(pBS216)。在抗性菌株的培养过程中，除儿茶酚-2,3-双加氧酶外，砷对萘生物降解关键酶的活性没有抑制作用。在包含植物-微生物联合体的模型系统中，菌株BS1393(pBS216, pKS1)和PCL1391(pBS216, pKS1)在存在砷的情况下能降解多达97%的添加萘。
