Institute for Animal Health, Pirbright Laboratory, Ash Road, Surrey, GU24 0NF, UK.
Vaccine. 2010 Apr 26;28(19):3428-34. doi: 10.1016/j.vaccine.2010.02.074. Epub 2010 Mar 1.
Previous work in cattle and pigs demonstrated that protection against foot-and-mouth disease (FMD) could be achieved following vaccination with chimeric foot-and-mouth disease virus (FMDV) vaccines, in which the VP1 G-H loop had been substituted with that from another serotype. This indicated that the VP1 G-H loop may not be essential for the protection of natural hosts against FMDV. If this could be substantiated there would be potential to develop FMD marker vaccines, characterised by the absence of this region. Here, we investigate the serological responses to vaccination with a virus with a partial VP1 G-H loop deletion in order to determine the likelihood of achieving protection and the potential of this virus as a marker vaccine. Inactivated, oil adjuvanted, vaccines, consisting of chemically inactivated virus with or without a partially deleted VP1 G-H loop, were used to immunise cattle. Serum was collected on days 0, 7, 14 and 21 and antibody titres calculated using the virus neutralisation test (VNT) to estimate the likelihood of protection. We predict a good likelihood that cattle vaccinated with a vaccine characterised by a partial VP1 G-H loop would be protected against challenge with the same virus containing the VP1 G-H loop. We also present evidence on the potential of such a construct to act as a marker vaccine, when used in conjunction with a novel serological test.
先前在牛和猪身上的研究工作表明，用嵌合口蹄疫病毒（FMDV）疫苗接种可以实现对口蹄疫（FMD）的保护，其中 VP1 G-H 环已被另一种血清型取代。这表明 VP1 G-H 环对于保护自然宿主免受 FMDV 的侵害可能不是必需的。如果这可以得到证实，那么开发 FMD 标记疫苗就有了潜力，其特征是缺少这一区域。在这里，我们研究了接种具有部分 VP1 G-H 环缺失的病毒后的血清学反应，以确定实现保护的可能性以及该病毒作为标记疫苗的潜力。使用化学灭活的病毒和/或具有部分缺失的 VP1 G-H 环的油佐剂灭活疫苗来免疫牛。在第 0、7、14 和 21 天收集血清，并使用病毒中和试验（VNT）计算抗体滴度，以估计保护的可能性。我们预测，接种具有部分 VP1 G-H 环的疫苗的牛很有可能在接种含有 VP1 G-H 环的相同病毒时受到保护。我们还提供了证据，证明在与新型血清学测试结合使用时，这种构建体有可能作为一种标记疫苗。
