Ranganathan Srikanth, Polshyna Anna, Nicholl Georgina, Lyons-Weiler James, Bowser Robert
Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA.
Clin Proteomics. 2006 Mar 1;2(1-2):91-101. doi: 10.1385/CP:2:1:91.
Recent studies have evaluated proper acquisition and storage procedures for the use of serum or plasma for mass spectrometry (MS)-based proteomics. The present study examines the proteome stability of human cerebrospinal fluid (CSF) over time at 23°C (room temperature) and 4°C using surface-enhanced laser desorption/ionization time-of-flight MS. Data analysis revealed that statistically significant differences in protein profiles are apparent within 4 h at 23°C and between 6 and 8 h at 4°C. Inclusion of protease and phosphatase inhibitor cocktails into the CSF samples failed to significantly reduce proteome alterations over time. We conclude that MS-based proteomic analysis of CSF requires careful assessment of sample collection procedures for rapid and optimal sample acquisition and storage.
最近的研究评估了用于基于质谱（MS）的蛋白质组学的血清或血浆的正确采集和储存程序。本研究使用表面增强激光解吸/电离飞行时间质谱，检测了人脑脊液（CSF）在23°C（室温）和4°C下随时间的蛋白质组稳定性。数据分析显示，在23°C下4小时内以及在4°C下6至8小时内，蛋白质谱存在统计学上的显著差异。在脑脊液样本中加入蛋白酶和磷酸酶抑制剂混合物并不能显著减少随时间推移的蛋白质组变化。我们得出结论，基于质谱的脑脊液蛋白质组学分析需要仔细评估样本采集程序，以实现快速且最佳的样本采集和储存。
