Lindquist Jeffrey N, Cheresh David A, Snyder Evan Y
The Burnham Institute for Medical Research, La Jolla, California, USA.
Curr Protoc Stem Cell Biol. 2010 Mar;Chapter 1:Unit 1F.9. doi: 10.1002/9780470151808.sc01f09s12.
The formation of the multicellular vascular system is critical to the growth, development, and viability of an organism, and many embryonic lethal mouse knockouts are due to vascular defects. Unfortunately, the complex nature, and many cell types involved in vasculogenesis and angiogenesis has stymied in vitro models of vascular formation. This unit describes a system that allows human embryonic stem cells to differentiate and spontaneously form vascular networks via both vasculogenesis and angiogenesis in the context of the three germ layers.
多细胞血管系统的形成对于生物体的生长、发育和生存能力至关重要，许多胚胎致死性小鼠基因敲除都是由于血管缺陷所致。不幸的是，血管生成和血管新生所涉及的复杂性质以及多种细胞类型阻碍了血管形成的体外模型的发展。本单元描述了一种系统，该系统能使人类胚胎干细胞在三胚层环境中通过血管生成和血管新生进行分化并自发形成血管网络。
