Codrea Vlad, Hayner Michelle, Hall Bradley, Jhaveri Sulay, Ellington Andrew
Department of Chemistry and Biochemistry, University of Texas, Austin, Texas, USA.
Curr Protoc Nucleic Acid Chem. 2010 Mar;Chapter 9:Unit 9.5.1-23. doi: 10.1002/0471142700.nc0905s40.
This unit describes the selection of aptamers from a single-stranded RNA pool that bind to small molecule targets. Aptamers generated by this type of selection experiment can potentially function as receptors for small molecules in numerous applications, including medical diagnostics, therapeutics, and environmental monitoring. This unit describes two modes of selection, one by column filtration and one by batch selection.
本单元介绍了从与小分子靶标结合的单链RNA库中筛选适体的方法。通过这类筛选实验产生的适体在众多应用中，包括医学诊断、治疗和环境监测，都有可能作为小分子的受体发挥作用。本单元描述了两种筛选模式，一种是通过柱过滤，另一种是通过批量筛选。
