Institute of Cell Biology, Zhejiang University, Hangzhou, People's Republic of China.
Anat Rec (Hoboken). 2010 May;293(5):858-64. doi: 10.1002/ar.21108.
TGF-beta-inducible early-response gene (TIEG) is a family of primary response genes induced by TGF-beta, which are well recognized in regulating cellular proliferation and apoptosis. However, their expression profile has never been investigated during embryogenesis in different organs. In this study, we aimed to investigate the transcriptional level of both TIEG1 and TIEG2 during development in various mice organs, including the brain cortex, cerebellum and stem, brain striatum, muscle, heart, liver, kidney, and lung. Quantitative real-time PCR was used to profile the change of transcriptional level of the two TIEG members in the mice tissues at six developmental stages. Taken together, the expression of TIEG1 and TIEG2 was specific in different organs yet varied with different developmental time points. Their dynamic changes were moderately consistent in most organs including the brain cortex, striatum, liver, kidney, and lung. However, their mRNA expression in both the heart and muscle was significantly different at all developmental stages, which might propose a compensation of functions in the TIEG family. Nevertheless, our data indicate that both the TIEG genes are essential in regulating the normal organ development and functioning in murine model, as their expressions were ubiquitous and tissue specific at various developmental stages.
TGF-β诱导的早期反应基因（TIEG）是 TGF-β诱导的主要反应基因家族，其在调节细胞增殖和凋亡方面得到了广泛认可。然而，它们在不同器官中的胚胎发生过程中的表达模式从未被研究过。在这项研究中，我们旨在研究 TIEG1 和 TIEG2 在各种小鼠器官（包括大脑皮层、小脑和干细胞、大脑纹状体、肌肉、心脏、肝脏、肾脏和肺）发育过程中的转录水平。定量实时 PCR 用于分析两个 TIEG 成员在小鼠组织中的转录水平在六个发育阶段的变化。总之，TIEG1 和 TIEG2 的表达在不同器官中具有特异性，但随着不同的发育时间点而变化。它们在大脑皮层、纹状体、肝脏、肾脏和肺等大多数器官中的动态变化是适度一致的。然而，它们在心脏和肌肉中的 mRNA 表达在所有发育阶段都有显著差异，这可能表明 TIEG 家族的功能存在代偿。然而，我们的数据表明，这两个 TIEG 基因在调节小鼠模型中的正常器官发育和功能方面都是必不可少的，因为它们在不同的发育阶段都具有普遍性和组织特异性。
