State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.
J Biomed Nanotechnol. 2009 Oct;5(5):591-5. doi: 10.1166/jbn.2009.1067.
With the existence of amino groups, chitosan (CS) possesses positive charge, so it can self-assemble with tripolyphotspate (TPP) possessing negative charge via ionic gelation. We here synthesized CS/TPP nanoparticles, and then characterized it with the infrared spectra (IR) and scanning electron microscope (SEM) and transmission electron microscopy (TEM) and used bovine serum albumin (BSA) as a model drug to investigate sustained-release properties of CS/TPP nanoparticles. Infrared spectrum of CS/TPP nanoparticles indicated that CS was cross-linked with TPP. A homogeneous size distribution and good dispersion and micro-porous surface of CS/TPP nanoparticles were observed. The highest encapsulation efficiency (EE) of the BSA in CS/TPP nanoparticles prepared in different concentration of CS reached 79.74%, while BSA continually and smoothly released up to one week.
壳聚糖（CS）由于存在氨基而带有正电荷，因此可以通过离子凝胶作用与带负电荷的三聚磷酸钠（TPP）自组装。我们在这里合成了 CS/TPP 纳米粒子，并通过红外光谱（IR）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）对其进行了表征，然后以牛血清白蛋白（BSA）为模型药物研究了 CS/TPP 纳米粒子的缓释性能。CS/TPP 纳米粒子的红外光谱表明 CS 与 TPP 发生了交联。观察到 CS/TPP 纳米粒子具有均匀的粒径分布、良好的分散性和微孔表面。在不同浓度的 CS 制备的 CS/TPP 纳米粒子中，BSA 的最高包封效率（EE）达到 79.74%，而 BSA 则持续且平稳地释放，直至一周。
