Jeganathan Aarthi, Arunachalam Karuppusamy, Byju Anju, Rani George Anju, Sajeev Sradha, Thangasamy Kavimani, Natesan Geetha
Department of Botany, Bharathiar University, Coimbatore 641046, TN, India.
Center for Studies in Stem Cells, Cellular Therapy and Toxicological Genetics (CeTroGen), Faculty of Medicine (FAMED), Federal University of Mato Grosso do Sul (UFMS), Campo Grande 79070-900, MS, Brazil.
Antioxidants (Basel). 2024 Dec 11;13(12):1513. doi: 10.3390/antiox13121513.
R.Br., a plant native to the Western Ghats, is recognized for its diverse medicinal properties. The plant's extracts, particularly rich in alkaloids and other bioactive compounds, have shown potential anticancer activity. This study investigates the therapeutic potential of chitosan nanoparticles (CNPs) loaded with the root methanolic extract (RME) of in combating breast cancer induced by dimethylbenz(a)anthracene (DMBA) in female Sprague Dawley rats. The RME-loaded chitosan nanoparticles (RME-EnCNPs) were synthesized and characterized, and their in vivo efficacy was evaluated. Treatment with RME-EnCNPs significantly inhibited tumor progression, which is evidenced by reduced tumor volume, burden, and incidence. Moreover, the nanoparticles demonstrated a sustained release of the active compounds, leading to marked improvements in various biochemical, enzymatic, and histopathological parameters. The study found that both RME and RME-EnCNPs effectively suppressed tumor growth, with RME-EnCNPs showing superior efficacy in modulating tumor progression. Antioxidant assays revealed that treatment with RME-EnCNPs (500 mg/kg) resulted in significant increases in total protein, superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), and glutathione (GSH) levels, alongside a marked reduction in lipid peroxidation (LPO) ( < 0.001). These findings suggest that RME-EnCNPs exert a potent antioxidant effect, mitigating oxidative stress within the tumor microenvironment. The root extract of and its nanoparticle formulation hold promise as a potential therapeutic agent for breast cancer, warranting further investigation to isolate active bioactive compounds and elucidate their mechanisms of action.
R.Br.是一种原产于西高止山脉的植物，因其多样的药用特性而闻名。该植物的提取物，特别是富含生物碱和其他生物活性化合物，已显示出潜在的抗癌活性。本研究调查了负载有R.Br.根甲醇提取物（RME）的壳聚糖纳米颗粒（CNPs）在对抗雌性Sprague Dawley大鼠中由二甲基苯并(a)蒽（DMBA）诱导的乳腺癌方面的治疗潜力。合成并表征了负载RME的壳聚糖纳米颗粒（RME-EnCNPs），并评估了它们的体内疗效。用RME-EnCNPs治疗显著抑制了肿瘤进展，这通过肿瘤体积、负担和发病率的降低得到证明。此外，纳米颗粒显示出活性化合物的持续释放，导致各种生化、酶学和组织病理学参数有显著改善。研究发现，RME和RME-EnCNPs均能有效抑制肿瘤生长，其中RME-EnCNPs在调节肿瘤进展方面显示出更优的疗效。抗氧化分析表明，用RME-EnCNPs（500毫克/千克）治疗导致总蛋白、超氧化物歧化酶（SOD）、过氧化氢酶、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽（GSH）水平显著增加，同时脂质过氧化（LPO）显著降低（P<0.001）。这些发现表明，RME-EnCNPs发挥了强大的抗氧化作用，减轻了肿瘤微环境中的氧化应激。R.Br.的根提取物及其纳米颗粒制剂有望成为乳腺癌的潜在治疗剂，值得进一步研究以分离活性生物活性化合物并阐明其作用机制。
