College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, China.
J Biomed Nanotechnol. 2009 Oct;5(5):602-6. doi: 10.1166/jbn.2009.1059.
A spectrofluorimetric method was established for the quantitative determination of 3-glycidoxypropyltrimethoxysilane (GPTMS), based on the quantitative reaction between dihydroxyl of hydrated epoxy groups and potassium periodate generating formaldehyde and the reaction of the formaldehyde with acetylacetone in the presence of ammonium ions generating a new compound having yellow green fluorescence. The established method was then used to determine the purity of a commercially obtained GPTMS sample and the amount of epoxy groups on GPTMS doped nanosilica composite (GPTMS/nano-SiO2). The conditions such as the concentrations of ammonium salts and acetylacetone, pH value, coloring temperature, and coloring time were investigated in detail with respect to their effects on the fluorescence intensity. It was found that the established method is simple in terms of operation and can be used to practically determine the purity of GPTMS and the amount of epoxy groups on GPTMS/nano-SiO2 with good accuracy and reproducibility.
建立了一种基于定量测定 3-缩水甘油丙基三甲氧基硅烷（GPTMS）的分光荧光法，该方法基于水合环氧基团的二羟基与高碘酸钾定量反应生成甲醛，以及在铵离子存在下甲醛与乙酰丙酮反应生成具有黄绿色荧光的新化合物。该方法用于测定商业获得的 GPTMS 样品的纯度和 GPTMS 掺杂纳米二氧化硅复合材料（GPTMS/nano-SiO2）上的环氧基团的量。详细研究了铵盐和乙酰丙酮的浓度、pH 值、显色温度和显色时间等条件对荧光强度的影响。结果表明，该方法操作简单，可用于实际测定 GPTMS 的纯度和 GPTMS/nano-SiO2 上的环氧基团的量，具有良好的准确性和重现性。
