Fox R A, Fernandez L A, Rajaraman R
Scand J Immunol. 1977;6(11):1151-7. doi: 10.1111/j.1365-3083.1977.tb00354.x.
Guinea pig peritoneal exudate cells were harvested 3 to 4 days after the intraperitoneal injection of Marcol oil. The washed cells were exposed to various concentrations of sodium periodate in phosphate-buffered saline (PBS) at pH 7.4 for 10 min at +4 degrees C. The cells were then used in the in vitro migration assay, and migration was consistently inhibited at concentrations from 10(-3) to 10(-5) M. The viability of the macrophages was not affected by this treatment. Sodium borohydride (10(-3) to 10(-5) M) in PBS for 10 min at pH 7.4 reversed the periodate effect. Experiments with purified macrophages showed that sodium periodate has a direct effect on macrophage function rather than an indirect effect via the potentiation of migration inhibition factor. In support of this, the in vitro spreading of macrophages on glass substrate for 1 h has been shown to be inhibited. This spreading inhibition can also be reversed by treatment with sodium borohydride. These results provide a new approach to understanding the biological significance and role of macrophage migration inhibition.
在腹腔注射马科尔油3至4天后，收集豚鼠腹腔渗出细胞。将洗涤后的细胞在4℃下于pH 7.4的磷酸盐缓冲盐水（PBS）中暴露于不同浓度的高碘酸钠10分钟。然后将这些细胞用于体外迁移试验，在10⁻³至10⁻⁵ M的浓度下迁移始终受到抑制。这种处理不影响巨噬细胞的活力。在pH 7.4的PBS中用硼氢化钠（10⁻³至10⁻⁵ M）处理10分钟可逆转高碘酸盐的作用。对纯化巨噬细胞的实验表明，高碘酸钠对巨噬细胞功能有直接影响，而非通过增强迁移抑制因子产生间接影响。为此，已证明巨噬细胞在玻璃基质上体外铺展1小时受到抑制。用硼氢化钠处理也可逆转这种铺展抑制。这些结果为理解巨噬细胞迁移抑制的生物学意义和作用提供了一种新方法。
