重排肌动蛋白结构对体外埃可病毒感染传播的作用。

Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro.

作者信息

Boratynska A, Martyniszyn L, Szulc L, Krzyzowska M, Szczepanowska J, Niemialtowski M G

机构信息

Division of Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.

出版信息

Acta Virol. 2010;54(1):41-8. doi: 10.4149/av_2010_01_41.

DOI:10.4149/av_2010_01_41

PMID:20201613

Abstract

We describe here a contribution of virus-induced actin tails and filopodia in transmission of Ectromelia virus (ECTV) infection in permissive cells detected by the immunofluorescence and confocal microscopy. Immunoblot analysis revealed profoundly decreased beta-actin levels during ECTV replicative cycle in the infected cells 24 hrs post infection (p.i.). These results provided a basis for the further analysis of ECTV motion in the infected cells as well as for impact of ECTV infection on the cytoskeletal proteins.

摘要

我们在此描述了病毒诱导的肌动蛋白尾和丝状伪足在允许性细胞中传播埃可病毒（ECTV）感染中的作用，这是通过免疫荧光和共聚焦显微镜检测到的。免疫印迹分析显示，在感染后24小时（p.i.）的感染细胞中，ECTV复制周期期间β-肌动蛋白水平显著降低。这些结果为进一步分析ECTV在感染细胞中的运动以及ECTV感染对细胞骨架蛋白的影响提供了基础。