Protein interaction matrix of Papaya ringspot virus type P based on a yeast two-hybrid system.

Comprehensive analysis of the interactions between 10 mature proteins of Papaya ringspot virus type P (PRSV-P) was carried out based on a yeast two-hybrid system assay (YTHS). We detected 6 interactions between different viral proteins (VPg-P1, VPg-P3, VPg-CI, VPg-CP, NIaPro-CI, and NIb-P3) and 4 self-interactions (HC-Pro, VPg, NIaPro, and CP). These interactions did not show the same directionality as corresponding interactions detected in other potyviruses and consequently, a protein interaction matrix displayed different patterns. This initial map of the protein interactions of PRSV-P allows further study of various viral proteins in order to develop anew strategy to control PRSV-P infection.