Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.
Fish Shellfish Immunol. 2010 Jul;29(1):94-103. doi: 10.1016/j.fsi.2010.02.020. Epub 2010 Mar 1.
Voltage-dependent anion channel (VDAC) proteins abound in the outer membrane of mitochondria. They play an important role in mitochondrial membrane permeabilization (MMP), which can lead to stress-induced cellular apoptosis and necrosis. Several pathogens regulate this MMP in their host cells to benefit their replication cycle, while in other cases, the host can use the same mechanism to combat pathogenesis. In this study, the first shrimp VDAC gene was identified and characterized from Marsupenaeus japonicus (MjVDAC). Its open reading frame (ORF) contained 849 bp encoding 282 amino acids. The deduced MjVDAC protein includes the 4-element eukaryotic porin signature motif, the conserved ATP binding motif (the GLK motif) and a VKAKV-like sequence known in other organisms to be involved in the protein's incorporation in the mitochondrial outer membrane. Tissue tropism analysis indicated that MjVDAC is abundant in the heart, muscle, stomach and pleopod. MjVDAC proteins colocalized with mitochondria in transiently transfected Sf9 cells and in shrimp hemocytes. dsRNA silencing of shrimp VDAC delayed white spot syndrome virus (WSSV) infection by 1 day in different shrimp organs. Taken together, these findings suggest that MjVDAC is likely to be involved in WSSV pathogenesis.
电压依赖性阴离子通道（VDAC）蛋白大量存在于线粒体的外膜中。它们在线粒体膜通透性（MMP）中发挥重要作用，MMP 可导致应激诱导的细胞凋亡和坏死。一些病原体在宿主细胞中调节这种 MMP，以有利于其复制周期，而在其他情况下，宿主可以利用相同的机制来对抗发病机制。在这项研究中，首次从日本对虾（Marsupenaeus japonicus）中鉴定并表征了虾类的第一个 VDAC 基因（MjVDAC）。其开放阅读框（ORF）包含 849 bp，编码 282 个氨基酸。推导的 MjVDAC 蛋白包含 4 元件真核孔蛋白特征基序、保守的 ATP 结合基序（GLK 基序）和在其他生物体中参与蛋白整合到线粒体外膜的 VKAKV 样序列。组织倾向性分析表明，MjVDAC 在心脏、肌肉、胃和腹肢中丰富表达。MjVDAC 蛋白在瞬时转染的 Sf9 细胞和虾血细胞中与线粒体共定位。虾 VDAC 的 dsRNA 沉默在不同虾器官中使白斑综合征病毒（WSSV）感染延迟了 1 天。总之，这些发现表明 MjVDAC 可能参与了 WSSV 的发病机制。
