State Key Joint Laboratory of Environment Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Bioresour Technol. 2010 Jul;101(14):5494-500. doi: 10.1016/j.biortech.2010.02.016. Epub 2010 Mar 3.
Microalgae have high potential to remove inorganic nutrients from wastewater and to produce biodiesel. Effects of nitrogen and phosphorus concentrations on growth, nutrient uptake, and lipid accumulation of a freshwater microalga Scenedesmus sp. LX1 were studied. Scenedesmus sp. LX1's growth was in accordance with the Monod model. The following Monod parameters were obtained: the N- and P-saturated maximum growth rate was 2.21 x 10(6) cells m L(-1)d(-1), and the half-saturation constants of N and P uptake were 12.1 mg L(-1) and 0.27 mg L(-1), respectively. In the nitrogen/phosphorus ratio of 5:1-12:1, 83-99% nitrogen and 99% phosphorus could be removed. In conditions of nitrogen (2.5 mg L(-1)) or phosphorus (0.1 mg L(-1)) limitation, Scenedesmus sp. LX1 could accumulate lipids to as high as 30% and 53%, respectively, of its algal biomass. The lipid productivity/unit volume of culture, however, was not enhanced. Further research should be made on how to enhance both lipid content and lipid productivity.
微藻具有从废水中去除无机营养物并生产生物柴油的巨大潜力。本研究考察了氮磷浓度对淡水微藻丽藻属 LX1 生长、养分吸收和油脂积累的影响。丽藻属 LX1 的生长符合 Monod 模型。得到了以下 Monod 参数：氮磷饱和最大生长速率为 2.21 x 10(6)细胞 mL(-1)d(-1)，氮和磷吸收的半饱和常数分别为 12.1 mg L(-1)和 0.27 mg L(-1)。在氮磷比为 5:1-12:1 时，可去除 83-99%的氮和 99%的磷。在氮（2.5 mg L(-1))或磷（0.1 mg L(-1))限制条件下，丽藻属 LX1 可分别将其藻细胞生物量的 30%和 53%积累为油脂。然而，单位培养体积的油脂生产力并没有提高。应进一步研究如何提高油脂含量和生产力。
