The Second Hospital of Nantong University, Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu 226001, China.
Vet J. 2011 Mar;187(3):374-80. doi: 10.1016/j.tvjl.2010.01.014. Epub 2010 Mar 3.
Neuronal nitric oxide synthase (nNOS) has been implicated in peripheral nerve lesions and regeneration. The CAPON adaptor protein interacts with the PDZ domain of nNOS, helping to regulate nNOS activity at post-synaptic sites in neurones, but it is not known whether its expression is altered in sciatic nerves after chronic nerve constriction injury. In the present study, the spatiotemporal expression of CAPON was determined in chronically constricted rat sciatic nerves. Similar to the level of protein expression, CAPON mRNA was significantly up-regulated for almost 5weeks following sciatic nerve injury. Immunohistochemistry demonstrated that increased CAPON was found mainly in S-100-positive Schwann cells. In addition, co-immunoprecipitation demonstrated an interaction between CAPON and nNOS in Schwann cells and the interaction was enhanced in injured sciatic nerves. CAPON may be involved in peripheral nerve regeneration through regulation of nNOS activity.
神经元型一氧化氮合酶（nNOS）已被牵涉到周围神经损伤和再生中。衔接蛋白 CAPON 与 nNOS 的 PDZ 结构域相互作用，有助于调节神经元突触后位点的 nNOS 活性，但尚不清楚其在慢性神经缩窄损伤后坐骨神经中的表达是否发生改变。在本研究中，确定了 CAPON 在慢性缩窄大鼠坐骨神经中的时空表达。类似于蛋白表达水平，CAPON mRNA 在坐骨神经损伤后近 5 周内显著上调。免疫组织化学显示，增加的 CAPON 主要存在于 S-100 阳性施万细胞中。此外，共免疫沉淀显示 CAPON 与施万细胞中的 nNOS 之间存在相互作用，并且在损伤的坐骨神经中这种相互作用增强。CAPON 可能通过调节 nNOS 活性参与周围神经再生。
