Gastroenterological Center, Yokohama City University Medical Center, 4-57, Urafune-cho, Minami-ku, Yokohama 232-0024, Japan.
Arch Virol. 2010 Apr;155(4):601-5. doi: 10.1007/s00705-010-0624-1. Epub 2010 Mar 5.
Hepatitis C virus (HCV) is the main causative agent of chronic liver disease, which may develop into liver cirrhosis and hepatocellular carcinoma. By using a recently developed reporter assay system in which genome-length HCV RNA replicates efficiently, we found that hydroxyurea (HU), a DNA synthesis inhibitor, inhibited HCV RNA replication. Moreover, we demonstrated that the anti-HCV activity of the combination of IFN-alpha and HU was higher than that of IFN-alpha alone. These results suggest that HU may be an effective anti-HCV reagent that can be used not only singly but also in combination with IFN-alpha to treat chronic hepatitis C.
丙型肝炎病毒（HCV）是慢性肝病的主要病原体，可能发展为肝硬化和肝细胞癌。我们利用最近开发的一种报告基因检测系统，该系统可有效地复制全长 HCV RNA，发现羟脲（HU），一种 DNA 合成抑制剂，可抑制 HCV RNA 复制。此外，我们证明 IFN-α与 HU 的联合抗 HCV 活性高于 IFN-α单独使用的活性。这些结果表明 HU 可能是一种有效的抗 HCV 试剂，不仅可以单独使用，还可以与 IFN-α联合用于治疗慢性丙型肝炎。
