Department of Biochemistry & Integrative Medical Biology, School of Medicine, Keio University, Tokyo, Japan.
Adv Exp Med Biol. 2010;662:101-7. doi: 10.1007/978-1-4419-1241-1_14.
Carbon monoxide (CO) is the stress-inducible gas generated by heme oxygenase (HO). Although the HO/CO system appears to contribute to cell protection and tissue repair under stress conditions, its mode of actions remains largely unknown. We hypothesized that CO might alter the cellular energetic conditions and thereby modulate oxygen metabolism. To examine this hypothesis, we attempted to establish a method to follow the global flux of (13)C-glucose in the cells using metabolomic approaches with liquid chromatography-mass spectrometry (LC-MS/MS). The human monoblastic leukemia cell line U937 was exposed to the CO-releasing molecule (CORM). The CO exposure attenuated the conversion of the mass-labeled glucose to its downstream metabolites, while significantly stimulating its conversion to those for pentose phosphate pathway, suggesting roles of stress-inducible CO in a shift of glucose biotransformation.
一氧化碳（CO）是血红素加氧酶（HO）产生的应激诱导性气体。尽管 HO/CO 系统在应激条件下似乎有助于细胞保护和组织修复，但它的作用机制仍在很大程度上未知。我们假设 CO 可能改变细胞的能量状态，从而调节氧代谢。为了检验这一假设，我们试图采用液相色谱-质谱联用（LC-MS/MS）代谢组学方法建立一种跟踪细胞中（13）C-葡萄糖整体通量的方法。用 CO 释放分子（CORM）处理人单核白血病细胞系 U937。CO 暴露减弱了质量标记葡萄糖向其下游代谢物的转化，同时显著刺激其向戊糖磷酸途径转化，表明应激诱导性 CO 在葡萄糖生物转化的转变中起作用。
