Department of Bio-System Engineering,Graduate School of Science and Engineering, Yamagata University, 992-0038 Yonezawa, Japan.
Adv Exp Med Biol. 2010;662:225-30. doi: 10.1007/978-1-4419-1241-1_32.
Near-infrared spectroscopy is a noninvasive optical technique used to monitor tissue oxygenation. Generally, the modified Beer-Lambert's law (MBL) using continuous-wave light has been used to measure active muscle oxygenation during exercise; however, it cannot measure absolute changes in the oxy- (oxy-[Hb + Mb]), deoxy- (deoxy-[Hb + Mb]), and total hemoglobin/myoglobin concentrations (total-[Hb + Mb]) because the pathlength and scattering coefficient are not measured. In contrast, the time-resolved spectroscopy (TRS) using a ultra short pulsed laser can be used to determine absolute changes in the concentration, although the temporal resolution is inferior to that in MBL. This study evaluated the absolute changes in active muscle oxygenation and the optical mean pathlength and scattering and absorption coefficient during high-intensity exercise by using the TRS system. In addition, the difference between the changes determined using TRS and MBL measurements was assessed. When the TRS and MBL measurements obtained during high-intensity exercise were compared, the total-[Hb + Mb] and oxy-[Hb + Mb] dynamics differed markedly during high-intensity exercise, while the deoxy-[Hb + Mb] dynamics and kinetics did not differ.
近红外光谱是一种用于监测组织氧合的非侵入性光学技术。通常，使用连续波光的修正 Beer-Lambert 定律（MBL）已用于测量运动期间活跃肌肉的氧合（氧合-[Hb + Mb]）、脱氧（脱氧-[Hb + Mb]）和总血红蛋白/肌红蛋白浓度（总-[Hb + Mb]）的变化；然而，由于未测量光程长度和散射系数，因此无法测量绝对值的变化。相比之下，使用超短脉冲激光的时间分辨光谱（TRS）可用于确定浓度的绝对值变化，尽管其时间分辨率劣于 MBL。本研究通过使用 TRS 系统评估了高强度运动期间活跃肌肉氧合以及光学平均光程长度和散射和吸收系数的绝对值变化。此外，还评估了使用 TRS 和 MBL 测量确定的变化之间的差异。当比较高强度运动期间获得的 TRS 和 MBL 测量值时，总-[Hb + Mb]和氧合-[Hb + Mb]动力学在高强度运动期间差异显著，而脱氧-[Hb + Mb]动力学和动力学没有差异。
