Centre for Nanoscience and Nanotechnology, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Victoria 3010, Australia.
Langmuir. 2010 Jun 15;26(12):9720-7. doi: 10.1021/la100430g.
Thin films exhibiting protein resistance are of interest in diverse areas, ranging from low fouling surfaces in biomedicine to marine applications. Herein, we report the preparation of low protein and cell binding multilayer thin films, formed by the alternate deposition of a block copolymer comprising polystyrene sulfonate and poly(poly(ethylene glycol) methyl ether acrylate) (PSS-b-PEG), and polyallylamine hydrochloride (PAH). Film buildup was followed by quartz crystal microgravimetry (QCM), which showed linear growth and a high degree of hydration of the PSS-b-PEG/PAH films. Protein adsorption studies with bovine serum albumin using QCM demonstrated that multilayer films of PSS/PAH with a terminal layer of PSS-b-PEG were up to 5-fold more protein resistant than PSS-terminated films. Protein binding was dependent on the ionic strength at which the terminal layer of PSS-b-PEG was adsorbed, as well as the pH of the protein solution. It was also possible to control the protein resistance of the films by coadsorption of the final layer with another component (PSS), which showed an increase in protein resistance as the proportion of PSS-b-PEG in the adsorption solution was increased. In addition, protein resistance could also be controlled by the location of a single PSS-b-PEG layer within a PSS/PAH film. Finally, the buildup of PSS-b-PEG/PAH films on colloidal particles was demonstrated. PSS-b-PEG-terminated particles exhibited a 6.5-fold enhancement in cell binding resistance compared with PSS-terminated particles. The stability of PSS-b-PEG films combined with their low protein and cell binding characteristics provide opportunities for the use of the films as low fouling coatings in devices and other surfaces requiring limited interaction with biological interfaces.
具有抗蛋白质特性的薄膜在多个领域都具有重要意义，从生物医学中的低污染表面到海洋应用都有涉及。在此，我们报告了由包含聚苯乙烯磺酸盐和聚（聚（乙二醇）甲基醚丙烯酸酯）（PSS-b-PEG）和聚烯丙胺盐酸盐（PAH）的嵌段共聚物交替沉积形成的低蛋白质和细胞结合的多层薄膜的制备。薄膜的构建过程通过石英晶体微天平（QCM）进行跟踪，结果表明 PSS-b-PEG/PAH 薄膜具有线性增长和高度水合作用。使用 QCM 进行牛血清白蛋白的蛋白质吸附研究表明，具有 PSS-b-PEG 末端层的 PSS/PAH 多层膜比 PSS 末端膜的抗蛋白质能力高 5 倍。蛋白质结合取决于吸附 PSS-b-PEG 末端层时的离子强度以及蛋白质溶液的 pH 值。通过与另一种成分（PSS）共同吸附最后一层也可以控制薄膜的抗蛋白质能力，随着吸附溶液中 PSS-b-PEG 的比例增加，蛋白质抵抗能力也随之增加。此外，通过 PSS/PAH 薄膜内单个 PSS-b-PEG 层的位置也可以控制蛋白质抵抗能力。最后，证明了 PSS-b-PEG/PAH 薄膜在胶体颗粒上的构建。与 PSS 末端颗粒相比，PSS-b-PEG 末端颗粒的细胞结合阻力提高了 6.5 倍。PSS-b-PEG 薄膜的稳定性结合其低蛋白质和细胞结合特性，为在需要与生物界面有限相互作用的设备和其他表面上使用这些薄膜作为低污染涂层提供了机会。
