Laboratory of Physical Chemistry, Department of Chemistry, Faculty of Science, University of Zagreb, Zagreb, Croatia.
Colloids Surf B Biointerfaces. 2010 Mar 1;76(1):305-10. doi: 10.1016/j.colsurfb.2009.11.009. Epub 2009 Dec 6.
The method of stagnation point optical reflectometry was applied for investigation of adsorption of bovine serum albumin (BSA) on previously formed poly(allylamine hydrochloride)/poly(sodium 4-styrenesulphonate) (PAH/PSS) multilayer with PAH being a terminal layer. The solid substrate was silica in the form of silicon wafers carrying an oxide layer. In order to interpret the adsorption of BSA, the build-up mechanism of PAH/PSS multilayers was examined with special emphasis on the effect of electrolyte concentration, pH of solution, and the anchoring (precursor) layer on that process. Additionally, the effect of BSA concentration and of the anchoring layer on BSA adsorption was investigated. It was shown that in all investigated systems the adsorption of BSA depends on conditions under which the multilayer was formed (ionic strength, pH and presence of an anchoring layer), as well as on BSA concentration. It follows that adsorption of BSA could be controlled not only by choosing suitable BSA concentration, but also by modifying the preformed multilayer.
停滞点光反射法被应用于研究牛血清白蛋白（BSA）在以前形成的聚烯丙基胺盐酸盐/聚（4-苯乙烯磺酸钠）（PAH/PSS）多层上的吸附，其中 PAH 是末端层。固体基质为硅片形式的氧化层载体。为了解释 BSA 的吸附，特别强调了电解质浓度、溶液 pH 值和锚固（前体）层对该过程的影响，检查了 PAH/PSS 多层的构建机制。此外，还研究了 BSA 浓度和锚固层对 BSA 吸附的影响。结果表明，在所研究的所有体系中，BSA 的吸附取决于形成多层的条件（离子强度、pH 值和锚固层的存在）以及 BSA 的浓度。因此，BSA 的吸附不仅可以通过选择合适的 BSA 浓度来控制，还可以通过修饰预先形成的多层来控制。
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