Department of Plant Pathology, Michigan State University, East Lansing, MI, USA.
Phytopathology. 2010 Apr;100(4):337-44. doi: 10.1094/PHYTO-100-4-0337.
A quantitative polymerase chain reaction assay was developed that could detect DNA of Rhabdocline pseudotsugae and R. oblonga among DNA of Douglas fir needles to a limit as low as three copies of target DNA. Differential infection rates of two varieties (seed sources) of Douglas fir interplanted in a field were studied in relation to staggered bud breaks. Infection of Douglas fir var. San Isabel corresponded to ascospore release times for Rhabdocline spp., whereas infection of var. Shuswap Lake did not occur throughout the spore release period during 2 years of study, despite abundant inoculum and adequate moisture during bud break. Rhabdocline spp. DNA was never detected in Shuswap Lake and disease symptoms were not observed in any year. We provide evidence that Shuswap Lake is resistant and probably immune to Rhabdocline spp. infection and Rhabdocline needlecast under Michigan conditions.
建立了一种定量聚合酶链反应检测方法，该方法能够在低至三个拷贝的目标 DNA 水平下检测到道格拉斯冷杉针中的拟叶松壳二孢菌和长形壳二孢菌的 DNA。为了研究错开芽期对两种道格拉斯冷杉（种源）的田间混交林的影响，研究了不同的感染率。圣伊莎贝尔变种的感染与拟叶松壳二孢菌的分生孢子释放时间相对应，而在 2 年的研究期间，尽管在芽期有充足的接种体和水分，但舒斯瓦普湖变种的感染并没有发生。在舒斯瓦普湖和任何一年都没有检测到拟叶松壳二孢菌的 DNA，也没有观察到任何症状。我们提供的证据表明，在密歇根州的条件下，舒斯瓦普湖对拟叶松壳二孢菌感染和拟叶松壳二孢菌叶枯病具有抗性，可能具有免疫力。
