生命早期摄入的α-亚麻酸较低会增加成年豚鼠的肥胖。
A low alpha-linolenic intake during early life increases adiposity in the adult guinea pig.
机构信息
Nestlé Research Centre, PO Box 44, Vers-Chez-Les-Blanc, 1000 Lausanne 26, Switzerland.
出版信息
Nutr Metab (Lond). 2010 Jan 29;7:8. doi: 10.1186/1743-7075-7-8.
BACKGROUND
The composition of dietary fatty acids (FA) during early life may impact adult adipose tissue (AT) development. We investigated the effects of alpha-linolenic acid (ALA) intake during the suckling/weaning period on AT development and metabolic markers in the guinea pig (GP).
METHODS
Newborn GP were fed a 27%-fat diet (w/w %) with high (10%-ALA group), moderate (2.4%-ALA group) or low (0.8%-ALA group) ALA content (w/w % as total FA) until they were 21 days old (d21). Then all animals were switched to a 15%-fat diet containing 2% ALA (as total FA) until 136 days of age (d136).
RESULTS
ALA and docosapentaenoic acid measured in plasma triglycerides (TG) at d21 decreased with decreasing ALA intake. Total body fat mass was not different between groups at d21. Adipose tissue TG synthesis rates and proliferation rate of total adipose cells, as assessed by 2H2O labelling, were unchanged between groups at d21, while hepatic de novo lipogenesis was significantly 2-fold increased in the 0.8%-ALA group. In older GP, the 0.8%-ALA group showed a significant 15-%-increased total fat mass (d79 and d107, p < 0.01) and epididymal AT weight (d136) and tended to show higher insulinemia compared to the 10%-ALA group. In addition, proliferation rate of cells in the subcutaneous AT was higher in the 0.8%-ALA (15.2 +/- 1.3% new cells/5d) than in the 10%-ALA group (8.6 +/- 1.7% new cells/5d, p = 0.021) at d136. AT eicosanoid profiles were not associated with the increase of AT cell proliferation.
CONCLUSION
A low ALA intake during early postnatal life promotes an increased adiposity in the adult GP.
背景
生命早期膳食脂肪酸（FA）的组成可能会影响成年脂肪组织（AT）的发育。我们研究了哺乳期/断奶期摄入α-亚麻酸（ALA）对豚鼠（GP）AT 发育和代谢标志物的影响。
方法
新生 GP 喂食 27%脂肪饮食（w/w%），其中 ALA 含量高（10%-ALA 组）、中（2.4%-ALA 组）或低（0.8%-ALA 组）（w/w%为总 FA），直至 21 天大（d21）。然后，所有动物都切换到含有 2%ALA（总 FA）的 15%脂肪饮食中，直到 136 天大（d136）。
结果
d21 时血浆三酰甘油（TG）中 ALA 和二十二碳五烯酸的测量值随 ALA 摄入量的减少而降低。d21 时各组间总体脂肪质量无差异。通过 2H2O 标记评估，脂肪组织 TG 合成率和总脂肪细胞增殖率在各组间无差异，而肝从头脂肪生成在 0.8%-ALA 组中显著增加了 2 倍。在较老的 GP 中，0.8%-ALA 组的总脂肪质量（d79 和 d107，p <0.01）和附睾脂肪组织重量（d136）显著增加了 15%，并且与 10%-ALA 组相比，胰岛素血症也有升高的趋势。此外，0.8%-ALA 组（5d 内新细胞 15.2 +/- 1.3%）比 10%-ALA 组（5d 内新细胞 8.6 +/- 1.7%）的皮下脂肪组织细胞增殖率更高（p = 0.021）。d136 时 AT 类二十烷酸谱与 AT 细胞增殖增加无关。
结论
生命早期低 ALA 摄入可促进成年 GP 的肥胖增加。
Zotero 插件